Methods for in vitro recombination

Details Methods for in vitro recombination Methods for in vitro recombination

1994-02-17

1997-02-25

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C12Q 168

Patent

active

056057938

ABSTRACT:
A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro of proteins.

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Feinberg and Vogelstein, Anal. Biochem. 132: pp. 6-13 (1983).
Horton, et al., Gene 77:pp. 61-68 (1989).
Ho, et al., Gene 77:pp. 51-59 (1989).
Pharmacia Catalog pp. 70-71 (1993 Edition).

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