Methods for the isolation and purification of the recombinantly

Details Methods for the isolation and purification of the recombinantly Methods for the isolation and purification of the recombinantly

1994-04-22

1996-11-26

Wax, Robert A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Lyase

435 691, 530417, C12N 988, C12N 1560

Patent

active

055784801

ABSTRACT:
This invention relates to methods for the isolation and purification of the recombinantly expressed major protein component of chondroitinase ABC, which is referred to as "chondroitinase I", from Proteus vulgaris (P. vulgaris). This invention further relates to methods for the isolation and purification of the recombinantly expressed second protein component of chondroitinase ABC, which is referred to as "chondroitinase II", from P. vulgaris. These methods provide significantly higher yields and purity than those obtained by adapting for the recombinant enzymes the method previously used for isolating and purifying native chondroitinase I enzyme from P. vulgaris.

REFERENCES:
patent: 5198355 (1993-02-01), Kikuchi et al.
Scopes (1982) Protein Purification. New York: Springer Verlag. pp. 197-199.
Yamagata et al. (1968) J. Biol. Chem 243: 1523-1535.

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