Method for optical determination of concentration of substance a

Radiant energy – Luminophor irradiation

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2504591, 436136, 436172, G01N 2164

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active

051516039

ABSTRACT:
The optical detection of the concentration of a specific substance in a liquid or a gas by the utilization of the quenching of a phosphor by the specific substance or a substance derived from the specific substance is attained with high accuracy by a method and apparatus which comprises causing the system of determination to induce the presence of a first phosphor (A) quenchable by the specific substance or a substance derived from the specific substance and a second phosphor (B) substantially unquenchable by the specific substance or a substance derived from the specific substance, measuring the light signal corresponding to the intensity (I.sup.f.sub.A) of emission from the first phosphor (A) and the light signal corresponding to the intensity (I.sup.f.sub.B) of emission from the second phosphor (B), and calculating the ratio between the light signals thereby offsetting variable factors relative to the intensities of emission. The first phosphor is selected from the group consisting of tris(2,2'-bipyridine) ruthenium (II) complex, tris(1,10'-phenanthroline) ruthenium (II) complex, 1-pyrenebutyric acid and salts thereof, pyrene, aminopyrine, perylene, perylene dibutylate, and 2, 7-dichlorofluorescein. The second phosphor is selected from the group consisting of proflavine sulfate, fluorescein, and eosin.

REFERENCES:
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patent: 4025393 (1977-05-01), Hirschfeld
patent: 4476870 (1984-10-01), Peterson et al.
patent: 4861727 (1989-08-01), Havenstein et al.
"Luminescence Ratio Indicators for Oxygen", Anal. chem. 1987 vol. 59, 279-283.
European Search Report 88 90 7814.
"Measurement of the Diffusion of Oxygen in Polymers by Phosphorescent Quenching", The Journal of Physical Chemistry, vol. 69, No. 11, Nov. 1965.
"Intracellular Measurement of Oxygen by Quenching of Fluorescence of Phyrenebutyric Acid", Biochim. Biophys. Acta, 279 (1972) 393-397.

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