Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification
Patent
1990-03-26
1992-09-29
Cashion, Jr., Merrell C.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Separation or purification
5303871, 5303891, 530412, C07K 318, C07K 328
Patent
active
051515040
ABSTRACT:
An improved method for the purification of monoclonal antibodies using Protein G, whereby the monoclonal antibodies are eluted from the Protein G at alkaline pH.
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FASEB Journal, vol. 3, No. 4, p. A1379 (1989) "Antibody Purification and Detection with Recombinant Protein G".
Jungbauer, A. et al., J. Chromatogr. 476, 257-268 (1989) "Comparison of Protein A, Protein G and Copolymerized Hydroxyapatite for the Purification of Human Monoclonal Antibodies".
Falkenberg, C. et al., Biomed. Chromatogr. 2, 221-225 (1987), "Purification of Streptococcal Protein G Expressed by Escherichia coli by High Performance Liquid Affinity Chromatography Using Immobilized Immunoglobulin G and Albumin".
Ohlson, S. et al., J. Immunol. Methods, 114, 175-180 (1988) "A Novel Approach to Monoclonal Antibody Separation using High Performance Liquid Affinity Chromatography (HPLAC) with SelecitSpher-10 Protein G".
Delacroix, D. et al., Molecular Immunology 16, 837-840 (1979) "Simple Purification of Goat IgG1 and IgG2 Subclasses by Chromatography on Protein A-Sepharose at Various pH".
Akerstrom, B. et al., J. Immunol. 135, 2589-2592 (1985) "Protein G: A Powerful Tool for Binding and Detection of Monoclonal and Polyclonal Antibodies".
Zymed Laboratories, Inc., (1989-1990) pp. 75-77 "Protein G Reagents" and pp. 89-90 Antibody Purification.
Bogden James M.
Cashion Jr. Merrell C.
E. R. Squibb & Sons Inc.
Rozycki Andrew G.
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