Human hybrid host cell for mammalian gene expression

Details Human hybrid host cell for mammalian gene expression Human hybrid host cell for mammalian gene expression

1998-12-10

2000-10-24

Chan, Christina Y.

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

435326, 435335, 435346, C12N 502, C12N 506

Patent

active

061365998

ABSTRACT:
Human/human hybrid cells were made via fusion of human embryonic kidney cells (293S) and modified Burkitt's lymphoma cells (2B8). The fusion cells are useful as host cells for the recombinant expression of mammalian genes. The advantages of using these hybrid clones of human kidney- and B-cells, called HKBs, for mammalian gene expression, include (i) the cells are negative for immunoglobulin expression, (ii) the cells grow easily in plasma protein-free medium (with or without the addition of recombinant insulin) as suspension cultures in a shake flask or in a fermenter (iii) the cells are very susceptible for transfection of DNA, and (iv) the cells secrete high levels of heterologous recombinant proteins, such as recombinant monoclonal antibodies, soluble ICAM-1, rIL-4, and rFVIII.

REFERENCES:
Stillman, et al. (Molec. & Cell. Biol., 1985; 2051-2060).
Peshwa, et al. (Biotech. & Bioeng., 1993; 179-187).
Walls, et al. (Gene, 1989; 139-149).
ATCC Cell Lines and Hybridomas (8th Ed., 1994; 149).

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