Silent inducible virus replicons and uses thereof

Details Silent inducible virus replicons and uses thereof Silent inducible virus replicons and uses thereof

1999-01-08

2000-10-24

Guzo, David

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

536 24, 536 1, 435 5, 435 691, 435 914, 435456, 435457, 435375, 4353201, 4241991, 42421811, 424 932, C12Q 168, C12Q 170, C07H 2104, A61K 4800

Patent

active

061365386

ABSTRACT:
A recombinant cell stably transformed with a cDNA of a silent, inducible replicon encoding a recombinant protein is disclosed. Transcription of the replicon cDNA is under the control of a silent promoter inducible by a DNA virus and expression of the recombinant protein is dependent upon the presence of the DNA virus in the cell. The cell can be engineered to package the replicon upon infection by the DNA virus, leading to intercellular amplification of expression of the recombinant protein. Where the recombinant protein is a reporter gene product, the recombinant cell may be used in an assay for detecting DNA viruses. A kit for performing such an assay is also provided.

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