Ion exchange chromatographic isoenzyme separation and isolation

Details Ion exchange chromatographic isoenzyme separation and isolation Ion exchange chromatographic isoenzyme separation and isolation

1976-01-30

1977-03-22

Shapiro, Lionel M.

Textiles: fiber preparation

1951035R, C07G 7026, C12K 100

Patent

active

040135136

ABSTRACT:
A process of separating and isolating fractions containing isoenzymes from a sample of tissue extract or blood serum comprising ion-exchange column chromatographically separating the sample containing the isoenzymes into a first fraction containing the hepatic isoenzyme of lactic acid dehydrogenase and a second fraction containing the myocardial isoenzymes of lactic acid dehydrogenase, the myocardial isoenzyme of creatine phosphokinase and prostatic acid phosphatase by ion-exchange column chromatographing the sample on a weakly-basic anion exchange chromatographic resin and sequentially eluting and collecting the first fraction and the second fraction.

REFERENCES:
nathan et al., Clinical Chemistry vol. 19, No. 9, pp. 1036-1039 (1973).
Nealon et al., Clinical Chemistry vol. 21, No. 3, pp. 392-397 (1975).
Mercer, Clinical Chemistry vol. 20, No. 1, pp. 36-40 (1974).

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