Enzyme-activated 1,2-dioxetanes

Details Enzyme-activated 1,2-dioxetanes Enzyme-activated 1,2-dioxetanes

1997-08-14

1999-02-09

Elliott, George C.

Organic compounds -- part of the class 532-570 series

Organic compounds

Heterocyclic carbon compounds containing a hetero ring...

252700, 568818, C07D32100, C07C 3522

Patent

active

058697055

ABSTRACT:
In an assay method in which a member of a specific binding pair is detected by means of an optically detectable reaction, the improvement wherein the optically detectable reaction includes the reaction, with an enzyme, of a dioxetane having the formula ##STR1## where T is a cycloalkyl or polycycloalkyl group bonded to the 4-membered ring portion of the dioxetane by a spiro linkage; Y is a fluorescent chromophore; X is H, alkyl, aryl, aralkyl, alkaryl, heteroalkyl, heteroaryl, cycloalkyl, cycloheteroalkyl, or enzyme-cleavable group; and Z is H or an enzyme-cleavable group, provided that at least one of X or Z must be an enzyme-cleavable group, so that the enzyme cleaves the enzyme-cleavable group from the dioxetane to form a negatively charged substituent bonded to the dioxetane, the negatively charged substituent causing the dioxetane to decompose to form a luminescent substance that includes group Y of said dioxetane.

REFERENCES:
patent: 4857652 (1989-08-01), Schaap
patent: 4962192 (1990-10-01), Schaap
patent: 4978614 (1990-12-01), Bronstein
patent: 5707559 (1998-01-01), Schaap et al.

Affiliated with

Also associated with

Rating

Enzyme-activated 1,2-dioxetanes does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Enzyme-activated 1,2-dioxetanes, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Enzyme-activated 1,2-dioxetanes will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1950113