Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1994-02-23
1996-09-03
Wax, Robert A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 15, 435 912, 4351721, 435810, 935 17, 935 77, 935 78, C12Q 168, C12Q 148, C21P 1934
Patent
active
055522750
ABSTRACT:
A method of human HLA-DR and/or Dw allotype matching comprises:
(i) effecting polymerase chain reaction (PCR) amplification of a HLA-DRB gene exon 2 nucleotide sequence of a first sample of DNA;
(ii) separating according to size the DNA fragments resulting from the said amplification;
(iii) determining the length polymorphism of the thus separated DNA fragments; and
(iv) comparing the thus-determined length polymorphism with the length polymorphism of DNA fragments resulting from PCR amplification of the said HLA-DRB gene exon 2 nucleotide sequence of a second sample of DNA.
REFERENCES:
patent: 4582788 (1986-04-01), Erlich
Bidwell et al., "A DNA-RFLP Typing System that Positively Identifies Serologically Well-defined and Ill-defined HLA-DR and DQ Alleles, Including DRw10," Transplantation, 45:640-646 (1988).
Wood et al., "HLA-DR Typing of Renal Allograft Patients and Donors by DNA-RFLP:Correlation with Pretransplant Serotyping," Transplantation Proceedings 22:2287 (Oct. 1990).
Mytilineos et al., "Comparison of RFLP-DR beta and Serological HLA-DR-typing in 1500 Individuals," Transplantation 59:870-873 (Nov. 1990).
Middleton et al., "Discrepancies in serological tissue typing revealed by DNA techniques," Transplant Int 1:161-164 (1988).
Clay et al., "PCR-SSO typing for DR4-Dw subtypes: application to unrelated bone marrow transplant donor selection," European J Immunogenetics, 18:97-104 (1991).
Uryu et al., "A simple and rapid method for HLA-DRB and -DQB typing by digestion of PCR-amplified DNA with allele specific restriction endonucleases," Tissue Antigens 35:20-31 (1990).
Bidwell et al., "Human HLA-DR/Dw allotype matching by analysis of HLA-DRB gene PCR product polymorphism (PCR fingerprints)," Technique 2:93-100 (1990).
Bohem et al., "HLA-DR beta genes vary in number between different DR specificities, whereas the number of DQ beta genes is constant," J Immunol 135:2149-2155 (1985).
Andersson et al., "Class II Genes of the Human Major Histocompatibility Complex," J Biol Chem 262:8748-8758 (1987).
Wood et al., "HLA-DR/Dw matching by PCR fingerprinting: the origin of PCR fingerprints and further applications," J Immunogenetic 18:147-153 (1991).
Scharf et al., "Sequence Analysis of the HLA-DRbeta and HLA-DQbeta Loci from Three Pemphigus vulgaris Patients," Human Immunology 22:61-69 (1988).
Ohmiya et al., Seperation of DNA Fragments by High-Resolution Ion-Exchange Chromatography on a Nonporous QA Column, Analytical Biochemistry, 189:126-130 (1990).
Katz and Dong, "Rapid Analysis and Purification of Polymerase Chain Reaction Products by High-Performance Liquid Chromatography," Bio Techniques, 8(5):546-555 (1990) .
Bidwell, et al., "Rapid HLA-DR-Dw and DP Matching by PCR Fingerprinting and Related DNA Heteroduplex Technologies," Handbook of HLA Typing Techniques, Chapter 3, p. 100-103 (1993), Hui & Bidwell, eds., CRC Press, Boca Raton, Fl.
Clay et al., "PCR-fingerprinting for selection of HLA matched unrelated marrow donors," Lancet, 337(8749):1049-1052 (May 4, 1991).
C. M. Nagamine et al., "A PCR Artifact:Geneation of Heteroduplexes" Amer. J. Human Genetics. 45:337-339 (Sep. 1989).
B. L. Triggs-Raine et al., "Diagnostic Heteroduplexes: Simple Detection . . . " Amer. J. Human Genetics 46:183 (Jan. 1990).
D. G. Tenen et al., "Effect of a Stem-Loop Structure within the Su40 . . . " Cell 34:629-639 (Sep. 1983).
Sekiya et al., "An Efficient Method of Detecting Nucleotide Sequence . . . " J. Cell Biochem. 13E:340.
Bidwell Jeffrey L.
Hui Kam M.
National University of Singapore
Prouty Rebecca
Wax Robert A.
LandOfFree
Human leukocyte antigen typing does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Human leukocyte antigen typing, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Human leukocyte antigen typing will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1949067