Process for the preparation of mature human growth hormone by en

Details Process for the preparation of mature human growth hormone by en Process for the preparation of mature human growth hormone by en

1994-02-28

1999-02-09

Naff, David M.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

435174, 435177, 435178, 530399, 930120, C12P 2106, C12N 1102, C12N 1110, A61K 3827, C07K 14475

Patent

active

058692789

ABSTRACT:
Mature Human Growth Hormone is prepared by enzymatic hydrolysis of a soluble precursor with immobilized Factor Xa. The soluble precursor contains a cleavage recognition sequence, Ile--Glu--Gly--Arg, for Factor Xa. The Factor Xa is preferably immobilized on CNBr activated Sepharose Cl-4B containing cyclic imido carbonate groups. Immobilization is carried out by reaction of amine groups on Factor X with the cyclic imido carbonate groups of the CNBr activated Sepharose Cl-4B to form covalently immobilized Factor X, which is then activated to Factor Xa with a protein contained in Russell's viper venom (RVV) in the presence of Ca.sup.++ ions.

REFERENCES:
patent: 5047333 (1991-09-01), Grandi et al.
Maugh (1984) Science, 223, 474-476, Guilbault et al. (1979) Acts. Chem. Res., 12, 344-350.
Imai et al. (1986) J. Biochem, 100(2), 425-432.
Takashi Imai et al., Journal of Biochemistry, 100, No. 2 (6038), Aug. 1986, "Synthesis and Characterization of Human Prorenin in Esherichia coli ", Tokyo, Japan, pp. 425-432.
Sylvia Ellinger et al., Journal of Clinical Microbiology, May, 1989, "Cleavage of Procaryotically Expressed Human Immunodeficiency Virus Fusion Proteins by Factor X.sub.3 and Application in Western Blot (Immunoblot) Assays", pp. 971-976.

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