Method for amplification and detection of RNA and DNA sequences

Details Method for amplification and detection of RNA and DNA sequences Method for amplification and detection of RNA and DNA sequences

1996-11-12

1999-02-09

Zitomer, Stephanie W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, 536 2433, 935 77, 935 78, C12Q 168, C12P 1934, C07H 2102

Patent

active

058692495

ABSTRACT:
A method of detecting and identifying specific human nucleic acid sequences contained in human nucleic acids in a human blood or tissue sample, comprising: (1) amplifying at least one portion of the specific nucleic acid sequence present in the sample; (2) transcribing the amplification product with an RNA polymerase to produce multiple RNA copies of each copy of specific nucleic acid sequence comprising the amplification product; and (3) identifying the transcription product.

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