Method of identifying compounds which modulate herpesvirus infec

Details Method of identifying compounds which modulate herpesvirus infec Method of identifying compounds which modulate herpesvirus infec

1996-01-05

1999-02-09

Wilson, James O.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 721, 435 736, 435 772, 435 15, C12Q 170, G01N 33567

Patent

active

058692347

ABSTRACT:
The present invention relates to a method of identifying a compound which modulates (enhances, inhibits, reduces) herpesvirus infection of a vertebrate cell comprising the steps of combining a phosphorylating enzyme capable of catalyzing the phosphorylation of ICP4, a substrate comprising a polypeptide which is phosphorylated by the enzyme, a phosphate source and the compound to be assessed; maintaining the combination under conditions appropriate for phosphorylation of the substrate; and determining phosphorylation of the substrate which occurs in the presence of the compound to be assessed. The present invention also relates to the compounds identified by the methods of the present invention.

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