Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1990-03-29
1993-06-08
Wityshyn, Michael G.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435291, 435817, 382 6, C12Q 102, C12M 134, G06K 900
Patent
active
052178757
DESCRIPTION:
BRIEF SUMMARY
BACKGROUND OF THE INVENTION
1. Field of the Invention
This invention relates to a method for detecting biological activities in a specimen, where the specimen and a culture medium are filled into a sealable container and are exposed to conditions enabling metabolic processes to take place in the presence of microorganisms in the sample, the concentration of initial substances being lowered and that of metabolic products being raised, and to a device for implementation of this method.
2. Description of the Prior Art
In many applications it must be possible to determine quickly whether a specimen is contaminated by microorganisms, such as bacteria, in particular in medical applications, in the pharmaceutical industry, food industry, or in environmental protection activities. The term "specimen" has a most comprehensive meaning here, including substances such as solid and liquid biological material (e.g., blood), food samples, such as frozen foods and preserved or canned foods, packaging material, clinical instruments and laboratory equipment, or samples taken from their surfaces, medical apparatus, first-aid and dressing material, soil and water samples, especially samples of drinking water.
For a long time, purely manual methods have been used in which the specimen to be assessed is put into a culture bottle containing a culture medium, and the growth of the culture is inspected only visually at given time intervals, and the type or presence of a microorganism is inferred from this observation.
In addition, some technical procedures and devices are known, with which the biological activities in a sample caused by microorganisms may be determined, and where the CO.sub.2 produced by the metabolism of the microorganism, or rather, the change in CO.sub.2 content, is employed as a measurement for determining the biological activity.
It is a known procedure, for instance, to bottle the sample to be assessed together with a radioactively labelled liquid culture medium and to test the atmosphere over the culture medium for radioactive gases, following which the presence of microorganisms in the simple may be determined.
Measuring systems of this kind are described in U.S. Pat. Nos. 3,676,679 and 3,935,073, for example. Although such systems are quick and reliable, they have certain disadvantages, i.e., radioactive substances must be handled and samples must repeatedly be taken from the gas space above the culture medium for continuous monitoring. When the samples are removed from the gas space, the remaining samples to be monitored may easily be contaminated by the sample-taking element and measuring errors may occur.
In European application 0 158 497 a system is disclosed in which the biological activity of a specimen is determined by means of infrared absorption. In this method a specimen is introduced into a sealable vessel containing a liquid culture medium, and is tested for the presence of microorganisms. The vessel is subjected to specific conditions, i.e., certain temperatures are maintained over given periods of time, thus enhancing the metabolism of the microorganisms, during which process CO.sub.2 is produced in the gas space above the culture medium by conversion of the carbon source. A sample is taken from the gas space and introduced into a measuring cell, and the CO.sub.2 content is measured by infrared absorption. Again, the subsequent samples may be contaminated, and another drawback is that infrared absorption is a less sensitive manner of measuring than radioactive labelling.
In order to avoid the problem of cross-contamination, the European application 0 104 463 proposes a method as well as a device of the kind mentioned in the introduction, which also are based on the detection of CO.sub.2 --produced by metabolic processes--by way of infrared absorption. In this method no sample is taken, but infrared radiation is directly transmitted through the wall of the vessel into the gas space above the culture medium, and its absorption is determined. Due to this non-invasive measuring method cross-con
REFERENCES:
patent: Re31879 (1985-05-01), Lubbers
patent: 4495293 (1985-01-01), Shaffar
MacFaddin, J. F., "Urease Test", Biochemical Tests for Identification of Medical Bacteria, The Williams & Wilkins Company, 1976, pp. 187-194.
MacFaddin, J. F., "Klinger's Iron Agar/Triple Sugar Iron Agar Tests", Biochemical Tests for Identification of Medical Bacteria, The Williams & Wilkins Company, 1976, pp. 108-118.
Karpf Hellfried
Smole Herbert
AVL AG
Williams Jane A.
Wityshyn Michael G.
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