Process and reagent for the determination of fibrin monomer in p

Details Process and reagent for the determination of fibrin monomer in p Process and reagent for the determination of fibrin monomer in p

1985-08-21

1987-12-01

Nucker, Christine M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving blood clotting factor

435214, 435217, 435810, 436 63, 436 69, C12Q 156, C12N 974, C12N 968

Patent

active

047104597

ABSTRACT:
The present invention provides a process for the determination of fibrin monomer in plasma, wherein a plasma sample containing fibrin monomer and freed from plasmin inhibitors is incubated in buffered solution with tissue plasminogen activator (EPA), plasminogen and a chromogenic plasmin substrate and the color formed is measured as a measure of the amount of fibrin monomer.
The present invention also provides a reagent for the determination of fibrin monomer in plasma, wherein it contains plasminogen, tissue plasminogen activator (EPA), chromogenic plasmin substrate and buffer (pH 7.0 to 9.0).

REFERENCES:
patent: 4429040 (1984-01-01), Becker et al.
patent: 4563420 (1986-01-01), Verheijen et al.
Ranby et al., Thrombosis Research, 27; 743-749, 1982.
Raanby et al, Prog. Fibrinolysis (1981), 5, 233-5 (abstract only, CA: vol. 98 (1983): 49234s).

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