Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues
Patent
1991-08-30
1995-02-21
Walsh, Stephen G.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
530825, 435 693, C07K 1300, C07K 1504
Patent
active
053917120
ABSTRACT:
Disclosed herein are variant versions of Streptolysin O produced by recombinant DNA techniques. In an embodiment, the variant is soluble upon expression and has a specific hemolytic activity of about 14 hemolytic units per milligram.
REFERENCES:
patent: 4925799 (1990-05-01), Rosenberg
Kehoe, M. & Timmus, K.N.; "Cloning and Expression in Escherichia coli, of the Streptolysin O Determinant from Streptococcus Pyogenes"; Infec. Imm. 43:804-810 (1984).
Kehoe, M. A. et al "Nucleotide Sequence of the Streptolysin O (SLO) Gene: Structural Homologies between SLO and Other Membrane-Damaging, Thiol-Activated Toxins"; Infect. Imm. 55:3228-3232 (Dec. 1987).
Becker, J. et al. "Nephelometric Determination of Antistreptolysin O." AACC 1991 Meeting, Washington, D.C. (Aug. 1, 1991).
Shatzman, A. R. & Rosenberg, M. "Expression Identification, and Chracterization of Recombinant Gene Products in Escherichia coli." Methods of Enzymology 152:661-675 (1987).
Shatzman, A. R. & Rosenberg, M. "The pAS Vector System and Its Application to Heterologous Gene Expression in Escherichia coli." Hepatology 7:305-355 (1987).
Degnen, G. A. & Cox E. C. "Conditional Mutator Gene in Escherichia coli: Isolation Mapping and Effector Studies." J. Bacter. 117:477-487 (1987).
Echols, H. et al. "Mutator Strains of Escherichia coli, mutD and dnaQ, with defective exonucleolytic editing by DNA polymerase III holoezyme." PNAS USA 80:2189-2192 (1983).
Schaaper, R. M. "Mechanisms of mutagenesis in the Escherichia coli mutator mutD5: Role of DNA mismatch repair." PNAS USA 85:8126-8130 (1986).
Alouf, J. E. "Streptococcal Toxins (Streptolysin O, Streptolysin S, Erythrogenic Toxin)". Pharmac. Ther. 11:661-717 (1980).
Geoffroy et al., "Alveolysin, The Thiol Activated Toxin . . . ", J. Bact. 172(12): 7301-7305, Dec. 1990.
Beckman Instruments Inc.
Harder P.R.
Henry Janis C.
May William H.
Walsh Stephen G.
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