Method of separating oligonucleotides from a mixture

Details Method of separating oligonucleotides from a mixture Method of separating oligonucleotides from a mixture

1994-05-10

1996-09-24

Elliott, George C.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C07H 2100

Patent

active

055592210

ABSTRACT:
A method of purifying full length synthetic target oligonucleotides from a mixture of oligonucleotides, particularly from a mixture containing truncated or failed sequences. The method involves attaching a short nucleotide sequence complementary to the 5' end of a target oligonucleotide to a solid support. The complementary between the most 5' nucleotides of the target oligonucleotide and the bound oligonucleotide results in hybridization which serves to retain the target oligonucleotide. Truncated or failed sequences lacking 5' sequences complementary to the attached oligonucleotide, fail to hybridize and therefore are not retained. The method makes it possible to purify gram quantities of synthetic deoxyribonucleic acids or ribonucleic acids and sequences which have modifications, such as on the phosphate backbone. The support-bound nucleotide sequences are stable under conditions of purification and therefore can be reused.

REFERENCES:
patent: 4358586 (1992-11-01), Rubin
Aviv et al., Proc. Natl. Acad. Sci. USA 69, 1408-1412 (1972).
Okamura et al., J. Biol. Chem. 253, 3765-3767 (1978).
Thanki et al., J. Biol. Chem. 253, 7740-7750 (1978).
Sheldon et al., Proc. Natl. Acad. Sci. USA 69, 417-421 (1972).

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