Methods for controlling electroosmotic flow in coated capillary

Details Methods for controlling electroosmotic flow in coated capillary Methods for controlling electroosmotic flow in coated capillary

1993-10-18

1995-02-21

Niebling, John

Chemistry: electrical and wave energy

Processes and products

Electrostatic field or electrical discharge

B01D 5702, B01D 6142

Patent

active

053912748

ABSTRACT:
Methods for controlling and varying electroosmotic flow in coated capillary electrophoresis columns under electrophoretic conditions are disclosed. The methods described herein involve varying the concentration of a multi-valent buffer compound in electrophoresis buffer compositions in order to control the electroosmotic flow in capillary columns having interior surfaces coated with charged organic coatings. Increasing the concentration of a multi-valent buffer compound which has a charge opposite the charge of the organic coating, in the electrophoresis buffer results in a decrease in electroosmotic flow. Decreasing the concentration of a multi-valent buffer compound results in an increase in electroosmotic flow. The ability to control electroosmotic flow provides enhanced resolution and optimizes separations of electrophoresis sample components.

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