Enhancement of microbial expression of polypeptides

Details Enhancement of microbial expression of polypeptides Enhancement of microbial expression of polypeptides

1983-08-10

1987-08-25

Wiseman, Thomas G.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

435 68, 4351723, 935 31, 935 42, C12P 2100, C12N 1500, C07H 1512

Patent

active

046894063

ABSTRACT:
Disclosed are novel DNA sequences operative as promoters of microbial transcription of structural genes and comprising synthetic E.coli bacteriophage T5 "early" promoter replica DNA sequences. Disclosed also are novel promoter/operator DNA sequences comprising a synthetic T5 early promoter replica DNA sequence associated with a regulatable operator DNA sequence providing for selectively regulatable transcription of structural genes in, e.g., E.coli cells transformed with a DNA vector including same.

REFERENCES:
Bujard et al., Promotors: Structure and Function (R. Rodriguez, M. Chamberlin, editors) 1982, pp. 121-140.
Gentz et al., "Cloning & Analysis of Strong Promotors is Made Possible by the Downstream Placement of an RNA Termination Signal", PNAS-USA-78(8), 4936-40, 1981.
Bujard, H. "Interaction of E. coli. RNA Polymerase with Promotors", TIBS(5)274-278, Oct. 1980.
Rommens et al., "Gene Expression: Chemical Synthesis & Molecular Cloning of a T5 Early Promotor", Nucleic Acids Research 11(17) 5921-40, 1983.
Sutcliff et al., "Plasmid Cloning Vectors": Chap. 4 in Genetic Engineering, edited by A. Chakrabarty, CRC Press, Inc., Fla., 1978.
Von Gabain et al., "Interaction of E. coli: RNA Polymerase w/Promotors of Corphage & Plasmid DNAS", PNAS VSA 76(1), 189-193, Jan. 1979.

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