Methods for attaching ligands or anti-ligands to a solid phase

Details Methods for attaching ligands or anti-ligands to a solid phase Methods for attaching ligands or anti-ligands to a solid phase

1984-09-21

1987-08-25

Kepplinger, Esther M.

Chemistry: analytical and immunological testing

Involving antibody fragments

436515, 436529, 436532, 436535, 436547, 436809, 436905, 427 2, G01N 33531

Patent

active

046893105

ABSTRACT:
Methods for attaching one component of a ligand-anti-ligand pair onto a solid phase surface. The component may be attached to a photoactivatable cross-linker capable of coupling the component to a colloidal medium coating the surface or alternatively, the component may be coupled to a bead and held in place against the surface by an overlay of the colloidal media having voids and spaces smaller than the diameter of the particle but large enough to permit diffusion of the other of the ligand-anti-ligand pair to be detected.

REFERENCES:
patent: 3966897 (1976-06-01), Renn
patent: 4542102 (1985-09-01), Dattagupta
Ji J. of Biological Chemistry 252(5) pp. 1566-1570 (1977).
Hackh's Chemical Dictionary 4th Edition, pp. 168 and 169, McGraw-Hill, New York (1980).
Lingwood Chemical Abstracts 102:2741r (1985).

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