Production method for PvuI restriction endonuclease

Details Production method for PvuI restriction endonuclease Production method for PvuI restriction endonuclease

Filed on

1989-12-14

Date issued

1991-09-17

Examiner

Schwartz, Richard A.

Patent Class

Chemistry: molecular biology and microbiology

SubClass

Enzyme , proenzyme; compositions thereof; process for...

SubSubClass

Hydrolase

Other Related Categories

435 691, 43525233, 4353201, 935 14, 935 73, C12N 922, C12N 121, C12N 1555, C12N 2100

Type

Patent

Status

active

Patent number

050495011

Description

ABSTRACT:
A recombinant vector comprising an incorporated chromosome DNA fragment containing a PvuI restriction enconuclease gene derived from Proteus vulgaris, a host transformed with the recombinant vector and a method of producing PvuI restriction endonuclease characterized in that the transformed host is cultivated and PvuI restriction endonuclease is harvested from the resulting culture. Since the host transformed with the recombinant vector of the present invention produces PvuI alone, it is unnecessary to remove PvuII in the purification process for PvuI, and further, since its productivity for nonspecific DNase is lower in comparison with conventional producer bacteria, DNase can be removed easily, making the production of PvuI easy.

REFERENCES:
Blumenthal, R. M. et al., 1985, "Cloning of a Restriction-Modification System from Proteus Vulgaris and Its Use in Analysing a Methylase-Sensitive Phenotype in Escherichia Soli", Journal of Bacteriology, vol. 164, pp. 501-509.
Wilson, G. G., 1988, "Cloned Restriction-Modification Systems-A Review", Gene, vol. 74, pp. 281-289.
T. R. Gingeras et al., "Two New Restriction Enconucleases from Proteus Vulgaris", Nucleic Acid Research, vol. 9 (18), 4525-4536 (1981).
K. D. Lunnen et al., "Cloning Type-II Restriction and Modification Genes", Gene, vol. 74, 25-32 (1988).
M. B. Mann et al., "Cloning of Restriction and Modification Genes in E. coli: The Hha II System from Haemophilus haemolyticus", Gene, vol. 3, 97-112 (1978).

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