Radioenzymatic method for assaying 3,4-dihydroxyphenylglycol

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving transferase

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23230B, 424 1, 435193, G01N 3348, C12N 910

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active

043117902

ABSTRACT:
A method for assaying 3,4-dihydroxyphenylglycol in a sample containing other catechol compounds by forming tritiated O-methylated derivatives, extracting tritiated 3-methoxy-4-hydroxyphenylglycol at a pH of 5 or lower, isolating the tritiated 3-methoxy-4-hydroxyphenylglycol and measuring its radioactivity. Prior to measurement, the tritiated 3-methoxy-4-hydroxyphenylglycol can be oxidized to tritiated vanillin which is extracted into the counting solution to increase specificity of the assay.

REFERENCES:
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Peuler et al., Life Sciences, vol. 21, 1977, pp. 625-636.
Saller et al., Life Sciences, vol. 23, 1978, pp. 1117-1130.
Martin et al., Chem. Abstracts, vol. 90, Apr. 9, 1979, Abstract No. 117415p.
Eriksson et al., Chem. Abstracts, vol. 88, Mar. 13, 1978, Abstract No. 71119y.

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