Method of extraction and purification of polyunsaturated fatty a

Details Method of extraction and purification of polyunsaturated fatty a Method of extraction and purification of polyunsaturated fatty a

1985-12-19

1988-12-20

Warren, Charles F.

Chemistry of carbon compounds

Miscellaneous organic carbon compounds

C-metal

260420, 2604285, 435134, C09F 500, C09F 510

Patent

active

047924187

ABSTRACT:
To extract and purify of polyunsaturated fatty acids, particularly EPA and DHA, without cis-trans conversion, from naturally occurring oils, such as marine animal oil and various vegetable oils a mixture of free fatty acids is first extracted from the oil. The resulting mixture of pure fatty acids is then subjected to separation by means of urea complexing to remove saturated fatty acids and most mono-unsaturated fatty acids. Remaining solvent and urea are then removed from the filtrate which is then subjected to low temperature fractional crystallization in the presence of an organic solvent such as acetone. Substantially pure polyunsaturated fatty acids, especially EPA and DHA, can be separately precipitated by reducing the temperature of the filtrate in two steps. Alternatively, the volume of the filtrate is reduced in two steps and cooled to form precipitates, which are removed. The final product is a mixture of substantially pure DHA and EPA.

REFERENCES:
patent: 4377526 (1983-03-01), Fujita et al.
Markley, Fatty Acids-Their Chemistry, Properties, Production and Uses, Part III, 1964, pp. 2309-2358, and newly cited pp. 2081-2104.
Teshima, S. et al. , "Separation of Polyunsaturated Fatty Acids by Column Chromatography on a Silver Nitrate-Impregnated Silica Gel", Bull. of Jap. Soc. of Sci. Fisheries, 44(8) 927 (1978).
Abu-Nasr, A. N. et al., J. Am. Oil Chemists Soc., 31, 41-45 (1954).
Swern, D. "Techniques of Separation: E. Urea Complexes", Fatty Acids: Their Chemistry, Properties, Production and Uses, edited by Klare S. Marklay, part 3, pp. 2309-2358, Interscience Publishers, N.Y., 1964.
Markley, K. S., "Techniques of Separation: A. Distillation, Salt Solubility, Low Temperature Crystallization", Fatty Acids, part 3, pp. 2081-2123, Interscience Publishers, (1964).
Privett, O. S. et al., J. Am. Oil Chemist. Soc., 36, 443-449 (1959).
Swern, D. et al., J. Am. Oil Chemist. Soc., 29, 431-434, 614-615 (1952).

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