Methods and compositions for the production of stably transforme

Details Methods and compositions for the production of stably transforme Methods and compositions for the production of stably transforme

1995-05-15

2000-02-15

Benzion, Gary

Multicellular living organisms and unmodified parts thereof and

Plant, seedling, plant seed, or plant part, per se

Higher plant, seedling, plant seed, or plant part

800300, 8003201, 800298, 536 241, 536 231, A01H 106, A01H 400, C12M 1500

Patent

active

060255455

ABSTRACT:
This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs the Streptomyces bar gene introduced by microprojectile bombardment into embryogenic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stables transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with the desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen.

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