Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1988-09-08
1989-12-19
Benson, Robert
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 32, 436501, 436503, 436518, 436531, 436815, 436819, G01N 3353
Patent
active
048882798
ABSTRACT:
New immunosorbent enzyme assays are provided employing antibiotic keying agents for binding a molecular species to be detected to a test surface followed by binding of an antibody specific for the species and detection of the antibody-species binding. In accordance with preferred embodiments, soluble peptidoglycan is assayed in a fluid binding it to a test surface with vancomycin, reacting the bound material with a peptidoglycan specific antibody, and measuring the extent of the antibody reaction. Assessment of bodily fluids and fermenation broths for the presence of certain kinds of antibiotics is one object of this invention. Assessment of sera for the presence of antibodies that have specificity for soluble peptidoglycans is another object of this invention. Assessment of bodily states in mammals is another object of the invention.
REFERENCES:
patent: 3852157 (1974-12-01), Rubenstein
patent: 4446231 (1984-05-01), Self
patent: 4486530 (1984-12-01), David
Strominger in .beta.-Lactam Antibiotics, Edited by Salton and Shockman, Academic Press, 1981, pp. 123-125.
Park, H. et al, "Detection of Soluble Peptidoglycan in Urine After Penicillin Administration", Infection and Immunity, pp. 139-142, Jan. 1984.
DePedro, M. A. et al., "Affinity Chromatography of Murein Precursors on Vancomycin-Sepharose", FEMS Microbiology Letters, 9, pp. 215-217 (1980).
Zeiger, A. R. et al., "Evidence for the Secretion of Soluble Peptidoglycans by Clinical Isolates of Staphylococcus Aureus", Infection and Immunity, pp. 1112-1118 (Sep. 1982).
Perkins, H., "Specificity of Combination Between Mucopeptide Precursors and Vancomycin or Restocetin", Journal Biochem, vol. XXX, pp. 195-205 (1968).
Heymer, B. et al., "A Latex Agglutination Test for Measuring Antibodies ot Streptococcal Mucopeptides", Journal of Immunology, vol. 111, No. 2, Aug. 1973, pp. 478-484.
Zeiger, A. et al., "Antibody Levels to Bacterial Peptidoglycan in Human Sera During the Time Course of Endocarditis and Bacteremic Infections Caused by Staphylococcus Aureus", Infection and Immunity, Sep. 1981, pp. 795-800, vol. 33, No. 3.
Park, H. et al., "Antibodies to Peptiodglycan in Patients with Spondylarthristis: A Clue to Disease Aetiology?", Annals of Rheumatic Diseases, vol. 43, pp. 725-728 (1984).
Zeiger, A. et al., "Antibodies Against a Synthetic Peptidoglycan-Precursor Pentapeptide Cross-React with at least Two Distinct Populations of Uncross-Linked Soluble Peptidoglycan Secreted by Mirococcus luteus Cells", Eur. J. Biochem, vol. 86, pp. 235-240 (1978).
Zeiger, A. et al., "Immunochemistry of a Synthetic Peptidoglycan-Precursor Pentapeptid", Biochemistry, vol. 12, No. 18, pp. 3387-3394 (1973).
Heymer, B. et al., "Detection of Antibodies to Bacterial Cell Wall Peptidoglycan in Human Sera", Journal of Immunology, vol. 117, No. 1, pp. 23-26, Jul. 1976.
Tynecka, Z. et al., "Peptidoglycan Synthesis in Bacillus Licheniformis The Inhibition of Cross-Linking by Benzylpenicillin and Cephaloridine in Vivo Accompanied by the Formation of Soluble Peptidoglycan", J. Biochem, vol. 146, pp. 253-267 (1975).
Heymer, B., "Biological Properties of the Peptidoglycan", Z. Immun--Forsch. Bd., vol. 149, pp. 245-257 (1975).
Kegleic, D. et al., "Isolation and Study of the Composition of a Peptidoglycan Complex Excreted by the Biotin-Requiring Mutant of Brevibacterium divaricatum NRRL-2311 in the Presence of Penicillin", Eur. J. Biochem, vol. 42, pp. 389-400 (1974).
Rosenthal, R. et al., "Evidence for the Synthesis of Soluble Peptidoglycan Fragments by Protoplasts of Streptococcus faecalis", Journal of Bacteriology, vol. 124, No. 1, pp. 398-409, Oct. 1975.
Mirelman, D. et al., "Penicillin-Induced Secretion of a Soluble, Uncross-Linked Peptidoglycan by Micrococcus luteus Cells", Biochemistry, vol. 13, No. 24, pp. 5045-5053 (1974).
Stewart-Tull, D. E. S., "The Immunological Activies of Bacterial Peptidoglycans", Ann. Rev. Microbiol, 34:311-40 (1980).
Methods in Immunology and Immunochemistry, Edited by Curtis A. Willliams, The Rockefeller University, New York, Academic Press, vol. II, Appendix I, pp. 343-364 (1968).
Arthritis & Rheumatism, vol. 21, No. 5 (Jun. 1978).
Ghuyse
Portions of this work were supported by Public Health Service grant AI 13525 from the National Institute of Allergy and Infectious Diseases.
Benson Robert
Thomas Jefferson University
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