Oligonucleotides for detecting bacteria and detection method usi

Details Oligonucleotides for detecting bacteria and detection method usi Oligonucleotides for detecting bacteria and detection method usi

1995-01-27

1996-05-14

Jones, W. Gary

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 231, 536 237, 536 2433, 435 6, 435 912, 435848, 435909, 935 8, 935 77, 935 78, C07H 2104, C12Q 168, C12N 100, C12N 1500

Patent

active

055168981

ABSTRACT:
Oligonucleotides (SEQ ID NOs 1-8) selectively hybridizable with a specific gene of Vibro parahaemolyticus, oligonucleotides (SEQ ID NOs 9-13) selectively hybridizable with the LT gene of toxigenic Escherchia coil, oligonucleotides (SEQ ID NOs 14-21) selectively hybrizable with the STh or STp gene of toxigenic Escherchia coil, oligonucleotides (SEQ ID NOs 22-47) selectively hybridizable with the entA, B, C, or D gene of Staphylococcus aureus, or oligonucleotides (SEQ ID NOs 48-53) selectively hybridizable with the entE gene of Staplyloccus aureus are prepared and used as primers for gene amplification to thereby selectively detect only respective microorganisms causing food poisoning.

REFERENCES:
Nishibuchi et al. Infect. Immun 57(9):2691-2697, 1989.
Nishibuchi et al Mol Microbiol 4(1) 87-100, 1990.

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