Chemistry: natural resins or derivatives; peptides or proteins; – Peptides of 3 to 100 amino acid residues – Synthesis of peptides
Patent
1991-02-22
1992-04-07
Russel, Jeffrey E.
Chemistry: natural resins or derivatives; peptides or proteins;
Peptides of 3 to 100 amino acid residues
Synthesis of peptides
210661, 210677, 210678, 422193, 422239, B01D 1200, B01D 1500, C07K 104, C07K 1704
Patent
active
051029863
DESCRIPTION:
BRIEF SUMMARY
This invention relates to a method of carrying out organic chemical reactions and apparatus for carrying out that method.
The invention has largely been developed for use in carrying out the synthesis of peptides but it will be understood that it is of broader application than that and can be used in carrying out a wide range of other organic chemical reactions.
In a known type of procedure, one of the substances that takes part in a reaction is attached to particles of a pervious support material so that at least some of the substance is within the particles. The material may be relatively rigid but it is preferred to use a soft gel. In carrying out the reaction, support material to which the substance is attached has a sequence of liquids applied to it, one after another. Some of the liquids are reagents which are used to bring about steps in the reaction process, while other liquids are solvents which are used for the washing or other treatment of the support material or of the substance attached to it. Liquids serving other purposes may also be employed. It is usual to place a quantity of the support material in a vessel which is open at the top and which has an outlet at the bottom leading to a discharge tube. A tap is provided adjacent to the outlet to enable the outlet to be opened and closed at will. Downstream of the tap a branch extends laterally from the discharge tube and can be connected to a vacuum pump. Inside the vessel, above the outlet, is a transverse plate make of sintered material; liquid can pass through the pores in the plate but the pores are too small to permit the particulate support material to pass through them.
In carrying out the method a first liquid is introduced into the vessel so that the support material becomes immersed in it. The vessel is shaken to allow the liquid to come into intimate contact with the particulate support material After a predetermined period of time has elapsed the tap is opened and the first liquid is drained off through the discharge tube; a vacuum is applied to the branch and assists the withdrawal of the liquid from the vessel. When this occurs, some of the liquid tends to remain trapped within the particles, and the particles themselves tend to pack together. In particular, if the particles are soft rather than rigid they tend to pack together to form a substantially impervious bed.
After as much as possible of the first liquid has been withdrawn, a second liquid is introduced into the vessel and the vessel is shaken again to allow the second liquid to come into contact with the support material. After a further predetermined period of time has elapsed the tap is re-opened and the second liquid is drained off in the same way that the first liquid was drained off. The process is then repeated with as many liquids as are required to complete the desired reaction or reactions.
As it is impossible to withdraw any of the liquids fully from the support material, each newly introduced liquid tends to be slightly contaminated with the previous liquid. This may lead to a reduced yield or to the need for the addition of extra steps in each of which the remaining unwanted liquid is washed by the introduction of a suitable liquid. An aim of the present invention is to enable those problems to be overcome or at least reduced.
From one aspect the present invention consists in a method of carrying out an organic chemical reaction in which a substance which takes part in the reaction is attached to particles of previous support material so that at least some of the substance is within the particles, a plurality of liquids is successively introduced into a vessel containing a quantity of that support material so that the material becomes immersed in each of those liquids successively and the reaction occurs as a consequence of the immersion of the quantity of support material in that succession of liquids, the introduction of at least one of the liquids into the vessel being carried out in such a manner that the immediately preceding liquid is progressively disp
REFERENCES:
patent: 4192798 (1980-03-01), Verlander et al.
patent: 4267056 (1981-05-01), McClure
patent: 4362699 (1982-12-01), Verlander et al.
patent: 4801449 (1989-01-01), Balint, Jr. et al.
Coffey Andrew F.
Epton Roger
Johnson Tony
Russel Jeffrey E.
Wolverhampton Polytechnic Higher Education Corporation
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