Signal processing method for analyzing autoradiograph

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250303, 36441301, 364496, 435 6, 436 94, 935 77, G06F 1542, G01N 3350

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049396676

ABSTRACT:
A signal processing method for analyzing an autoradiograph by subjecting digital image data containing an autoradiograph of a resolved pattern to digital signal processing to obtain locational information on radioactively labeled substances in the form of numerals and/or symbols is disclosed. The resolved pattern is formed by resolving radioactively labeled substances, such as, for example, nucleic acids, in a one-dimensional direction on a support medium. The total boundary area of the resolved patterns throughout the digital image data is determined by first dividing the digital image data into two or more blocks along a direction perpendicular to the resolving direction and then preparing a waveform composed of positions along the direction perpendicular to the resolving direction for each block by compiling the digital image date for each block. The waveform is used to establish the boundary of the area of the resolved pattern for each block, and the boundaries of the resolved pattern area of each block are interpolated to establish the total boundary area of the resolved patterns. This method is an easily conducted, accurate analytical method for obtaining locational information and, thus, the identity of a wide variety of radioactively labeled substances.

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