Method involving display of protein binding pairs on the surface

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 5, 435 732, 435 697, 4351723, 4353201, 536 234, C12Q 168, C12Q 170, C12P 2104, G01N 33554

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active

055166377

ABSTRACT:
The present invention relates to a fusion protein, comprising a pilin protein or a portion thereof and a heterologous polypeptide (target protein). In a preferred embodiment it relates to a method for displaying the target protein on the outer surface of a bacterial host cell capable of forming pilus. In certain embodiments, it is desirable that the pilus is a receptor for bacteriophage attachment and infection. The F pilus is preferred.

REFERENCES:
patent: 5223409 (1993-06-01), Ladner et al.
patent: 5348867 (1994-09-01), Georgiou et al.
Duenas et al (1994) "Intra- and Extracellular expression of an scFv antibody fragment in E. coli: Effect of bacterial strains and" etc. BioTechniques 16(3)476-483.
Harrison et al (1990) "Presentation of foreign antigenic determinants at the bacterial cell surface using the TraT lipoprotein" Res. Microbiol. 141:1009-1012.
Soderlind et al (1992) "Phage display technology in antibody engineering: Design of phagemid vectors and in vitro maturation systems." Immun. Rev. 130:109-124.
Little et al. (1994) "Surface display of antibodies" Biotech. Adv. 12:539-555.
L. S. Frost and W. Paranchych, MGG 1988, 213:134-139.
K. A. Ippen-Ihler and E. G. Minkley, Jr., Ann. Rev. Genet. 1986, 20:593-624.
G. Georgiou, et al., TIBTECH, Jan. 1993 (vol. 11) 6-10.
W. D. Paiva, et al., J of Biol. Chem., vol. 267, No. 36, pp. 26191-26197 (1992).
T. Grossman, et al., J. of Bacteriology, Mar. 1990, pp. 1174-1179, vol. 172 No. 3.
M. Hoefnung, Methods in Cell Biology, vol. 34, pp. 77-105 (1991).
J. K. Scott, Techniques, 1992 pp. 241-245.
P. Fuchs, et al; Biotechnology vol. 9 Dec. 1991 pp. 1370-1372.
Grussenmeyer et al. (1985) "Complexes of polyoma virus medium T antigen and cellular proteins" Proc. Natl. Acad. Sci. USA 82:7952-7954.
Newton et al., Immune Response to Cholera Toxin Epitope Inserted in Salmonella Flagellin, Science, vol. 244:70-72.
Frost, et al., A Sequence of The F traALE Region that Includes the Gene for F Pilin, Journal of Bacteriology, vol. 160:395-402, Oct. 1994.
Frost, et al., Characterization and Sequence Analysis of Pilin from F-Like Plasmids, Journal of Bacteriology, vol. 164:1238-1247.
McCafferty, et al., Phage antibodies: filamentous phage displaying antibody variable domains, Letters to Nature, vol. 348:552-554.
Thiry et al. (1989) "Cloning of DNA sequences encoding foreign peptides and their expression in the K88 pili." Applied and Environmental Microbiology 55(4):984-993.
Yamagishi et al. (1990) "Mutational analysis of structure-activity relationships in human tumor necrosis factor alpha." Protein Engineering 3(8):713-719.
Duenas et al. (1994) "Clonal selection and amplification of phage displayed antibodies by linking antigen recognition and phage replication" Bio/Technology 12:999-1002.
Barbas et al (1991) Assembly of combinatorial antibody libraries on phage surfaces: The gene III site. Proc. Natl. Acad. Sci. USA 88:7978-7982.

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