Primer directed nucleic acid amplification including the additio

Details Primer directed nucleic acid amplification including the additio Primer directed nucleic acid amplification including the additio

1991-05-02

1996-05-14

Fleisher, Mindy

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 6, C12P 1934, C12Q 168

Patent

active

055162924

ABSTRACT:
Methods for the enzymatic reduction and/or elimination of contaminant DNA from enzymatic amplification procedures are disclosed. These methods include the use of restriction endonucleases, DNAases and exonucleases which act upon contaminant DNA in polymerase chain reaction mixtures such that contaminant DNA is not amplified.

REFERENCES:
patent: 4800159 (1989-01-01), Mullins et al.
patent: 5427929 (1995-06-01), Richards et al.
Strom et al. (1989), Am. J. Hum. Genet. 45 (Suppl. 4), A221.
Furrer et al. (Jul. 1990), Nature 346, 324.
Sambrook et al. (1989), Molecular Cloning: A Laboratory Manual, 2nd Ed. (Cold Spring Harbor Press), pp. 14.2, 14.15, 5.3-5.9.

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