Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Patent
1981-01-30
1984-09-04
Kepplinger, Esther M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
435 6, 435 29, 4351721, C12Q 170, C12Q 168, C12Q 102, C12N 1500
Patent
active
044697864
ABSTRACT:
The present application discloses a short-term microbial assay useful in detecting chemical mutagens. A Bacillus subtilis which is infected with a strain of the temperate bacteriophage .phi.105 having a conditional lethal mutation is specifically illustrated. In the assay exogenous DNA from wild-type .phi.105 is reacted with a potential mutagen and then transfected into the B. subtilis carrying a mutated .phi.105 DNA. After plating, an increase in the frequency of clear plaques signifies that the test compound is mutagenic.
The present invention provides a short-term mutagenicity test which detects frameshift as well as base substitution mutations induced by chemicals.
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Garro Anthony J.
Phillips Robert A.
Kepplinger Esther M.
The Mount Sinai School of Medicine of the City University of New
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