Substrate formulation in 2-amino-2-methyl-1-propanol buffer for

Details Substrate formulation in 2-amino-2-methyl-1-propanol buffer for Substrate formulation in 2-amino-2-methyl-1-propanol buffer for

1986-01-08

1988-05-31

Marantz, Sidney

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435 4, 435 7, 435188, 436805, C12Q 142, G01N 33535

Patent

active

047481153

ABSTRACT:
A stable substrate formulation for an alkaline phosphatase assay includes 5-bromo-4-chloro-3-indolyl phosphate, nitro blue tetrazolium and a 2-amino-2-methyl-1-propanol buffer. Addition of MgCl.sub.2 to this formulation promotes stability.

REFERENCES:
patent: 4030995 (1977-06-01), Starkweather
patent: 4563417 (1986-01-01), Albarella et al.
Leary, Jeffrey J. et al., "Rapid and Sensitive Colorimetric Method for Visualizing Biotin-labeled DNA probes hydridized to DNA or RNA immobilized on nitrocellalose:Bio blots", PNAS (80), pp. 4045-4049, Jul. 1983.

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