Imaging method for scanning microscopes, and confocal scanning m

Details Imaging method for scanning microscopes, and confocal scanning m Imaging method for scanning microscopes, and confocal scanning m

1990-10-22

1992-01-28

Nelms, David C.

Radiant energy

Photocells; circuits and apparatus

Optical or pre-photocell system

2502013, H01J 314

Patent

active

050846124

ABSTRACT:
Preliminary two-dimensional scanning of a sample with a light beam is carried out before an operation for obtaining an image signal representing a microscope image is carried out. During the preliminary scanning of the sample, a direction and an amount of a deviation in position of the light beam, which has passed through the sample, from the position of a pinhole of a pinhole plate, are detected for each position which is being scanned with the light beam irradiated to the sample. A storage device stores information about the direction and the amount of the deviation and the information about the corresponding position, which is being scanned. During the operation for obtaining the image signal from the sample, for which the preliminary two-dimensional scanning has been carried out, the pinhole plate is moved for each position, which is being scanned, in accordance with the information about the direction and the amount of the deviation, which information is stored on the storage device and which corresponds to each position which is being scanned.

REFERENCES:
patent: 4827125 (1989-05-01), Goldstein
patent: 4845352 (1989-07-01), Benschop
patent: 4877960 (1989-10-01), Messerschmidt et al.

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