Purified mixture of collagenase I, collagenase II and two other

Details Purified mixture of collagenase I, collagenase II and two other Purified mixture of collagenase I, collagenase II and two other

1996-01-24

1998-05-19

Saucier, Sandra E.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435219, C12N 952, C12N 950

Patent

active

057534853

ABSTRACT:
A purified enzyme mixture useful for isolating cells or cell clusters from tissue is disclosed. The mixture includes at least two collagenase enzymes, at least two other proteases, and additional non-protease components. The mixture is purified by removing at least some of the non-protease components. The purified mixture may then be used to isolate cells or cell clusters from tissue. Also disclosed are the essential components of the purified enzyme mixture as well as preferred ranges and ratios of these essential components for isolating cells or cell clusters from tissue. Finally, a dissociation system is disclosed that can be used with the purified enzyme mixture to dissociate tissue and recover cells or cell clusters.

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