Phenylalanyl tRNA synthetase polynucleotides of streptococcus

Details Phenylalanyl tRNA synthetase polynucleotides of streptococcus Phenylalanyl tRNA synthetase polynucleotides of streptococcus

1997-04-18

1998-05-19

Wax, Robert A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

435 6, 4353201, 4352523, 43525411, 435325, 536 232, 536 234, C12N 900, C12N 120, C12N 1500, C07H 2104

Patent

active

057534799

ABSTRACT:
The invention provides pheS (beta) and pheS (alpha) polypeptides and DNA (RNA) encoding pheS (beta) and pheS (alpha) polypetides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing pheS (beta) and pheS (alpha) polypeptides to screen for antibacterial compounds.

REFERENCES:
R. Calendar et al., "Purification and Physical Characterization of Tyrosyl Ribonucleic Acid Synthetases form Escerichia coli and Bacillus subtilis", Biochemistry, 5(5) pp. 1681-1690 (1966).
J. Hughes et al., "How Does Pseudomonas Fluorescens, the Producing Organism of the Antibiotic Pseudomonic Acid A, Avoid Suicide?", FEBS Letters, 122(2) pp. 322-324 (1980).
Brakhage et al., "Structure and Nucleotide Sequence of the Bacillus subtilis Phenylalanyl-tRNA Synthetase Genes," Biochime, 72 pp. 725-734 (1990).
Kreutzer R. et al., "Structure of the Phenylalanyl-tRNA Synthetase Genes from Thermus thermophilus HB8 and Their Expression in E. coli," Nucleic Acids Research, 20(1) pp. 4173-4178 (1992).
Fleischmann et al. (1995) Whole-Genome Random Sequencing and Assembly of Haemophilus influenza Rd. Science 269:496-512.

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