Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1997-08-13
2000-06-20
Brusca, John S.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 911, 435 913, 4353201, 536 231, 536 243, 536 2431, 536 2432, 536 2433, 536 253, 536 2532, C12P 1934, C12Q 168, C07H 2102, C07H 2104
Patent
active
060776684
ABSTRACT:
The present invention provides detectably labeled RNA and DNA oligonucleotide multimers useful as diagnostic probes in medical, biological and chemical applications. A method for synthesizing DNA and RNA oligonucleotides, oligonucleotide multimers, and analogs, preferably those that are detectably labeled, is also provided. Oligonucleotide synthesis is performed by combining a circular single-stranded oligonucleotide template with an effective polymerase and at least two types of nucleotide triphosphate, without the addition of auxiliary proteins, to yield an oligonucleotide multimer comprising multiple copies of a repeated oligonucleotide sequence.
REFERENCES:
patent: 4795700 (1989-01-01), Dervan et al.
patent: 4837312 (1989-06-01), Dervan et al.
patent: 4987071 (1991-01-01), Cech et al.
patent: 5093246 (1992-03-01), Cech et al.
patent: 5246921 (1993-09-01), Reddy et al.
patent: 5258506 (1993-11-01), Urdea et al.
patent: 5354668 (1994-10-01), Auerbach
patent: 5354855 (1994-10-01), Cech et al.
patent: 5426180 (1995-06-01), Kool
patent: 5470724 (1995-11-01), Ahern
patent: 5498531 (1996-03-01), Jarrell
patent: 5500357 (1996-03-01), Taira et al.
patent: 5714320 (1998-02-01), Kool
Uhlmann et al. Antisense Oligonucleotides: A New Therapuetic Principle. Chemical Reviews vol. 90(4):544-584, Jun. 1990.
Uhlen. Magnetic seperation of DNA. Nature vol. 340:733, Aug. 31, 1989.
Kawai et al. A Simple Method of Detecting Amplified DNA with Immobilized Probes on Microtiter Wells. Analytical Biochemistry vol. 209:63-69, Feb. 15, 1993.
Ledwith et al. Preperation of Synthetic Tandem-Repetitive probes for DNA Fingerprinting. Biotechniques vol. 9(2):149-152, 1990.
May et al. DNA Fingerprinting by Specific Priming of Concatenated Oligonucleotides. Nucleic Acids Research vol. 19(16):4557, 1991.
Ali S. Enzymatic Synthesis of DNA Probes Complementary to a Human Variable Number Tandem Repeat Locus. Analytical Biochemistry vol. 179:280-283, 1989.
"Affinity Chromatography: Practical and Theoretical Aspects", Ed. P. Mohr; Dekker Publishing; New York (1985); Title page, Copyright page, and Contents pages (pp. v-viii).
Aguilar, L. et al., "Hairpin, Dumbbell, and Single-Stranded Phosphodiester Oligonucleotides Exhibit Identical Uptake in T. Lymphocyte Cell Lines", Antisense & Nucleic Acid Drug Development, 6, 157-163 (1996).
Aiyar, S. E. et al., "A Mismatch Bubble in Double-stranded DNA Suffices to Direct Precise Transcription Initiation by Escherichia coli RNA Polymerase", J. Biol. Chem., 269(18), 13179-13184 (1994).
Albrecht, T. et al., "Cationic lipide mediated transfer of c-abl and bcr antisense oligonucleotides to immature normal myeloid cells: Uptake, biological effects and modulation of gene expression", Ann. Hematol., 72, 73-79 (1996).
Ashley, G.W., et al., "Chemical Synthesis of Oligodeoxynucleotide Dumbbells", Biochemistry, 30, 2927-2933 (1991).
Beaucage, S.L., et al., "Deoxynucleoside Phosphoramidites--A New Class of Key Intermediates for Deoxypolynucleotide Synthesis", Tetrahedron Lett., 22(20), 1859-1862 (1981).
Beaucage, S.L., et al. "The Functionalization of Oligonucleotides Via Phosphoramidite Derivatives," Tetrahedron, 49(10), 1925-1963 (1993).
Blanco, L. et al., "Highly Efficient DNA Synthesis by the Phage .phi.29 DNA Polymerase", J. Biol. Chem., 264(15), 8935-8940 (1989).
Bledsoe, A. H. et al., "Molecular Homology and DNA Hybridization," J. Mol. Evol., 30, 425-433 (1990).
Bock, L.C., et al., "Selection of single-stranded DNA molecules that bind and inhibit human thrombin", Nature, 355, 564-566 (1992).
Capaccioli, S. et al., "Cationic Lipids Improve Antisense Oligonucleotide Uptake and Prevent Degradation in Cultured Cells and in Human Serum", Biochemical and Biophysical Research Communications, 197(2), 818-825 (1993).
Chin, J., et al., "Catalytic Hydrolysis of Amides at Neutral pH", J. Chem. Soc., Chem. Commun., 1326-1328 (1990).
Compton, J., "Nucleic acid sequence based amplification", Nature, 350, 91-92 (1991).
Cwirla, S. E. et al., "Peptides on phage: A vast library of peptides for identifying ligands", PNAS USA, 87, 6378-6382 (1990).
Daube, S. S. et al., "Functional Transcription Elongation Complexes from Synthetic RNA-DNA Bubble Duplexes", Science, 258, 1320-1324 (1992).
Daubendiek, S. L., et al., "Rolling-Circle RNA Synthesis: Circular Oligonucleotides as Efficient Substrates for T7 RNA Polymerase", J. Am. Chem. Soc., 117, 7818-7819 (1995).
D'Souza, D. J. et al., "Strong Binding of Single-stranded DNA by Stem-Loop Oligonucleotides", J. Biomolecular Structure and Dynamics, 10(1), 141-152 (1992).
Dzianott, A. M., et al., "Derivation of an infectious viral RNA by autolytic cleavage of in vitro transcribed viral cDNAs", PNAS USA, 86, 4823-4827 (1989).
Egholm, M. et al., "PNA hybridizes to complementary oligonucleotides obeying the Watson-Crick hydrogen-bonding rules," Nature, 365, 566-568 (1993).
Eisenberg, S. et al., "The Single-Stranded DNA Phages", Eds., Denhardt, D.T., Cold Spring Harbor Press, Cold Spring Harbor, Title page, Copyright Page, Contents Pages, pp. 298-299 (1978).
Eisenberg, S., et al., "Enzymatic replication of viral and complementary strands of duplex DNA of phage .phi.X174 proceeds by separate mechanisms", PNAS USA, 73(9), 3151-3155 (1976).
Ellington, A. D., et al., "In vitro selection of RNA molecules that bind specific ligands", Nature, 346, 818-822 (1990).
Ellington, A. D., et al., "Selection in vitro of single-stranded DNA molecules that fold into specific ligand-binding structures", Nature, 355, 850-852 (1992).
Famulok, M., et al., "Stereospecific Recognition of Tryptophan Agarose by in vitro Selected RNA", J. Am. Chem. Soc., 114, 3990-3991 (1992).
Fire, A., et al., "Rolling replication of short DNA circles", PNAS USA, 92, 4641-4645 (1995).
Forster, A.C. et al., "Structural and Ionic Requirements for Self-cleavage of Virusoid RNAs and trans Self-cleavage of Viroid RNA", Cold Spring Harbor Symposia on Quantitative Biology, LII, 249-259 (1987).
Grosshans, C. A., et al., "A hammerhead ribozyme allows synthesis of a new form of the Tetrahymena ribozyme homogenous in length with a 3' end blocked for transesterification", Nucleic Acids Research, 19(14), 3875-3880 (1991).
Gura, T., "Antisense Has Growing Pains", Science, 270, 575-577 (1995).
Guy-Caffey, J. K., et al., "Novel Polyaminolipids Enhance the Cellular Uptake of Oligonucleotides", J. Biol. Chem., 270(52), 31391-31396 (1995).
Harshey, et al., "A mechanism of DNA transposition", PNAS USA, 78, 1090-1094 (1981).
Haseloff, J., et al., "Simple RNA Enzymes with New and Highly Specific Endoribonuclease Activities", Nature, 334, 585-591 (1988).
Horn, T. et al., "The Synthesis of Branched Oligonucleotides as Signal Amplification Multimers for Use in Nucleic Acid Assays," Nucleosides & Nucleotides, 8(5&6), 875-877 (1989).
Hutchins, C. J., et al., "Self-cleavage of plus and minus RNA transcripts of avocado sunblotch viroid", Nucleic Acids Research, 14(9), 3627-3641 (1986).
James, W., "Towards gene-inhibition therapy: a review of progress and prospects in the field of antiviral antisense nucleic acids and ribozymes", Antiviral Chemistry & Chemotherapy, 2(4), 191-214 (1991).
Kanaya, E., et al., "Template-Directed Polymerization of Oligoadenylates Using Cyanogen Bromide", Biochemistry, 25, 7423-7430 (1986).
Kazakov, S., et al., "A Trinucleotide Can Promote Metal Ion-Dependent Specific Cleavage of RNA", Pro. Natl. Acad. Sci. USA, 89, 7939-7943 (1992).
Kim, J. H., et al., "Dimethyl Phosphate Hydrolysis at Neutral pH", J. Am. Chem. Soc., 114, 9792-9795 (1992).
Kitajima, I., et al., "Ablation of Transplanted HTLV-I Tax-Transformed Tumors in Mice by Antisense Inhibition of NF-.kappa.B", Science, 258, 1792-1795 (1992).
Koo, Hyeon-Sook, et al., "Determination of the Extent of DNA Bending by an Adenine-Thymine Tract", Biochemistry, 29, 4227-4234 (1990).
Kool, E., "Molecular Recognition by Circular Oligonucleotides: Increasing the Selectivity of DNA Binding", J. Am. Chem. Soc., 113, 6265-6266 (1991).
Kool, E. T., et al., "Abstract of National Institute of Health Grant No. R01-GM46625" (prior to Feb. 1997).
Kool, E.T., " Binding of HIV
Brusca John S.
McGarry Sean
University of Rochester
LandOfFree
Highly sensitive multimeric nucleic acid probes does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Highly sensitive multimeric nucleic acid probes, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Highly sensitive multimeric nucleic acid probes will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1850816