Concentrated immunoglobulin solution suited for intravenous admi

Details Concentrated immunoglobulin solution suited for intravenous admi Concentrated immunoglobulin solution suited for intravenous admi

1980-01-07

1982-03-09

Hazel, Blondel

Drug, bio-affecting and body treating compositions

Lymphokine

424 87, 260112B, A61K 39395

Patent

active

043189024

ABSTRACT:
A process for the preparation of an immunoglobulin solution containing IgM in concentrated form and suited for intravenous administration, comprising treating an IgM-containing protein fraction obtained by conventional fractionation from blood plasma or serum with .beta.-propiolactone in an amount such that the ratio of .beta.-propiolactone to a 5% solution of the IgM-containing proteins is from about 0.05 to 0.15 ml per 100 ml. Advantageously, prior to the treatment with .beta.-propiolactone the IgM-containing protein fraction is freed of lipids by treatment with colloidal silica gel and with crosslinked dextrans or diethylaminoethyl cellulose. The starting material used is a Cohn fraction III of human blood plasma which has been dissolved in physiological saline solution to a concentration of about 5% protein, the treatment with .beta.-propiolactone is carried out at about 20.degree. to 37.degree. C. for about 4 to 6 hours until a substantially constant pH of about 8 is obtained, and the solution is thereafter sterile filtered.

REFERENCES:
patent: 3597409 (1971-08-01), Breuer
Chemical Abstracts, vol. 71, No. 11, 1969, p. 187, Abstract No. 47731v, Stephan, W., "Elimination of Complement Fixation in .gamma.-Globulin by Chemical Modification with .beta.-Propiolactone".
Van Der Hoven, A., et al., Immunochemistry, vol. 10, pp. 107-114, 1973.
Chemical Abstracts, vol. 78, No. 17, Apr. 30, 1973, Abstract No. 109110x, p. 314, A. Van Der Hoven et al.; "Isolation of Immunogenically Pure IgM from Cohn Fraction III of Pools Normal Human Plasma".

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