Chemistry: molecular biology and microbiology – Apparatus – Bioreactor
Patent
1998-03-05
2000-05-23
Beisner, William H.
Chemistry: molecular biology and microbiology
Apparatus
Bioreactor
4352841, 4352861, 4352865, 4352972, C12N 300
Patent
active
060664959
ABSTRACT:
Apparatus and methods are disclosed for maturing an elongate replacement tissue construct in vitro prior to use of the replacement construct in vivo as, for example, a ligament. The tissue is seeded with specific cells, exposed to a maturation fluid, and subjected to selected forces, which can include longitudinal stress, (i.e. stressing the tissue along its elongate axis). The tissue is disposed in a maturation chamber that confines maturation fluid for introduction to the tissue. A first mounting element couples to a first end of the elongate biopolymer tissue and a second mounting element couples to a second end of the tissue such that the tissue extends along a longitudinal axis, and a force is applied to at least one of the mounting elements for longitudinally stressing the tissue. The foregoing apparatus and methods are intended to provide a replacement tissue that is more readily integrable in vivo, i.e., a tissue that more readily degrades, regenerates and remodels in vivo to produce a more durable and functional replacement tissue.
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Kanda et al. `Behavior of Arterial Wall Cells Cultured on Periodically Stretched Substrates.` Cell Transplantation. vol. 2 (1993), pp. 475-484.
D. Huang et al., "Mechanisms and Dynamics of Mechanical Strengthening in Ligament-Equivalent Fibroblast-Populated Collagen Matrices," Annals of Biomedical Engineering, vol. 21, pp. 289-305 (1993).
E. Bell, Tissue Engineering: Current Perspectives, Birkhauser, Boston, 1993 .
Bell Eugene
Fofonoff Timothy W.
Beisner William H.
Laurentano Anthony A.
Tissue Engineering, Inc.
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