Modified green fluorescent proteins

Details Modified green fluorescent proteins Modified green fluorescent proteins

1996-11-20

2000-05-23

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435440, 530350, 530855, C12P 2104, C12N 1500, C07K 100

Patent

active

060664762

ABSTRACT:
Modifications in the sequence of Aequorea wild-type GFP provide products having markedly different excitation and emission spectra from corresponding products from wild-type GFP. In one class of modifications, the product derived from the modified GFP exhibits an alteration in the ratio of two main excitation peaks observed with the product derived from wild-type GFP. In another class, the product derived from the modified GFP fluoresces at a shorter wavelength than the corresponding product from wild-type GFP. In yet another class of modifications, the product derived from the modified GFP exhibits only a single excitation peak and enhanced emission relative to the product derived from wild-type GFP.

REFERENCES:
patent: 5264563 (1993-11-01), Huse
patent: 5491084 (1996-02-01), Chalfie
patent: 5625048 (1997-04-01), Tsien et al.
patent: 5741668 (1998-04-01), Ward et al.
patent: 5777079 (1998-07-01), Tsien et al.
Heim et al., "Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy tranfer," Current Biology 6(2): 178-182 (1996).
Inouye and Tsuji, "Expression of the gene and fluorescence characteristics of the recombinant protein," FEBS Letters 341: 277-280 (1994).
Mitra et al., "Fluorescence resonance energy transfer between blue-emitting and red-shifted excitation derivatives of the green fluorescent protein," Gene 173(1): 13-17 (Jul. 1, 1996).
Roth, Thesis from the Graduate Program in Biochemistry from Rutgers, the State University of New Jersey (Oct. 1985).
Baldwin et al., Biochemistry 29:5509-9915 (1990).
Chalfie et al., Science 263:802-805 (1994).
Cody et al., Biochemistry 32:1212-1218 (1993).
Cubitt et al., Trends in Biochem. Sci. 20:488-455 (1995).
Delagrave et al., Bio/Technology 13:151-154 (1995).
Deschamps et al., Protein Expression and Purification, 6:555-558 (1995).
Ehrig et al., FEBS Letters 367:163-166 (1995).
Heim et al., Proc. Natl. Acad. Sci. U.S.A. 91:12501-12505 (1994).
Heim et al. Nature 373:663-664 (1995).
Kain et al, Bio Techniques 19:650-655 (1995).
Levine et al., Comp. Biochem. Physiol. 728:77-85 (1982).
Muhlrad et al., Yeast 8:79-82 (1992).
Norris et al., Plant Molecular Biology, 24:673-677 (1994).
Prasher et al., Gene 111:229-233 (1992).
Sala-Newby et al, Biochem. J. 279:727-732 (1991).
Surpin et al., Photochem. Photobiol. 49:Abstract, 25S (1989).
Ward, in Bioluminescence and Chemiluminescence (eds. DeLuca et al., 235-242 (Academic Press, New York, 1981).
Ward et al., Biochemistry 21:4535-4540 (1982).
Ward et al., Photochem. Photobiol. 35:803-808 (1982).
Wilbanks et al., J. Biol. Chem. 268:1226-1235 (1993).

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