Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1998-04-29
2000-05-23
Campbell, Eggerton A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 912, C12Q 168, C12P 1934
Patent
active
060664576
ABSTRACT:
Nucleic acid starting material composed of a collection of single stranded nucleic acid molecules is anchored and processed by a direction random printing method to produce a mixture of shorter random size DNA molecules well-suited for achieving substantially uniform global PCR amplification. The processing and global amplification method disclosed is especially useful in conjunction with subtractive hybridization procedures applied, for example, to the study of differential gene expression.
REFERENCES:
patent: 5545522 (1996-08-01), Van Gelder et al.
Patanjali, S.R., et al., Construction of a Uniform-Abundance (Normalized CDNA Library, Proceedings of the National Academy of Sciences of USA, Mar. 1, 1991, vol. 88, No. 5, pp. 1943-1947, XP000368687.
Hampson I.N. et al., Chemical Cross Linking Subtraction (CCLS): A New Method for the Generation of Subtractive Hybridisation Probes, Nucleic Acids Research, 1992, vol. 20, No. 11, p. 2899 XP002050204.
Sharma, P. et al., PCR-Based Construction of Subtractive CDNA Library Using Magnetic Beads, Biotechniques, Oct. 1, 1993, vol. 15, No. 4, pp. 610,612 XP000402905.
Ermolaeva, O.D. et al., Subtractive Hybridization, A Technique for Extraction of DNA Sequences Distinguishing Two Closely Related Genomes: Critical Analysis, Genetic Analysis: Biomolecular Engineering, Jul. 1996, vol. 13, No. 2, pp. 49-58 XP000635153.
Hampson , I.N., et al., Directional Random Oligonucleotide Primed (DROP) Global Amplification of CDNA: Its Application to Subtractive CDNA Cloning, Nucleic Acids Research, 1996, vol. 24, No. 23, pp. 4832-4835.
Hampson Ian Noel
Hampson Lynne
Campbell Eggerton A.
Cancer Research Campaign - Technology Limited
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