Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1995-07-07
1997-11-04
Chambers, Jasemine C.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 912, 536 243, 536 2431, 536 2433, C12Q 168, C07H 2104, C12P 1934
Patent
active
056838808
ABSTRACT:
The present invention is directed to a method for rapidly analyzing whether known genetic markers which are found in various lengths in the population, e.g., those containing (CA).sub.n repeats, are associated with a disease of interest. The method involves using polymerase chain reactions to amplify the DNA in the marker regions and comparing the amplified DNA lengths seen in the normal population with those seen in an affected population of persons. The method involves a pooling of DNA samples from normal patients to average out the normal marker genotype found in the population and also involves a pooling of DNA from affected individuals to give a summing effect to give a stronger signal. The amplified DNA fragments are labeled with two distinguishable markers such as two different colored fluorescent markers, one used to label the amplified DNA from the normal population and the other to label the amplified DNA from the affected population. The amplified products from the normals and the affecteds are pooled, run on a sequencing gel, and a difference spectrum is calculated. Markers which are not associated with the disease will result in a zero or near zero difference spectrum whereas a marker which is associated with the disease will result in a difference spectrum with marked peaks.
REFERENCES:
patent: 5364759 (1994-11-01), Caskey et al.
LeDuc, C. et al. (1995). "Batched Analysis of Genotypes," PCR-Methods And Applications 4:331-336.
Jeffrey, A.J. (1985). "Hypervariable `minisatellite` regions in human DNA," Nature 314:67-73.
Nakamura, Y. et al. (1987). "Variable Number of Tandem Repeat (VNTR) Markers for Human Gene Mapping," Science 235:1616-1622.
Saiki, R.K. et al. (1985). "Enzymatic Amplification of .beta.-Globin Genomic Sequences and Restriction Site Analysis for Diagnosis of Sickle Cell Anemia," Science 230:1350-1354.
Hunkapiller, T. et al. (1991). "Large-Scale and Automated DNA Sequence Determination," Science 254:59-67.
Smith, L.M. et al. (1986). "Fluorescence detection in automated DNA sequence analysis," Nature 321:674-679.
Mullis, K.B. and Faloona, F.A. (1987). "Specific Synthesis of DNA in Vitro via a Polymerase-Catalyzed Chain Reaction," Methods in Enzymology 155:335-350.
Saiki, R.K. et al. (1988). "Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase," Science 239: 487-491.
Litt, M. and Luty, J.A. (1989). "A Hypervariable Microsatellite Revealed by In Vitro Amplification of a Dinucleotide Repeat within the Cardiac Muscle Actin Gene," Am. J. Hum. Genet. 44:397-401.
Weber, J.L. and May, P.E. (1989). "Abundant Class of Human DNA Polymorphisms Which Can Be Typed Using the Polymerase Chain Reaction," Am. J. Hum. Genet. 44:388-396.
Botstein, D. et al. (1980). "Construction of a Genetic Linkage Map in Man Using Restriction Fragment Length Polymorphisms," Am. J. Hum. Genet. 32:314-331.
Arnheim et al., "Use of pooled DNA samples to detect linkage disequilibrium of polymorphic restriction fragments and human disease: Studies of the HLA class II loci." Proc. Natl. Acad. Sci. USA 82: 6970-6974 Oct. 1985.
Sheffield et al., "Identification of a Bardet-Biedl syndrome locus on chromosome 3 and evaluation of an efficient approach to homozygosity mapping", Hum. Molec. Genet, 3: 1331-1335. Aug. 1994.
Carmi et al., "Use of a DNA pooling strategy to identify a human obesity syndrome locus on chromosome 15", Hum. Molec. Genet. 4:9-13. Jan. 1995.
Khatib et al., "Determining relative microsatellite allele frequencies in pooled DNA samples", PCR Meth. Appl. 4: 13-18. 1994.
Chambers Jasemine C.
Myriad Genetics Inc.
Priebe Scott D.
LandOfFree
Linkage analysis of genes with diseases using difference spectru does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Linkage analysis of genes with diseases using difference spectru, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Linkage analysis of genes with diseases using difference spectru will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1832257