Method for detecting a target nucleic acid analyte in a sample

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 5, 435 911, 435 912, 4351739, 536 243, 536 2432, 536 2433, 210360, 42218603, C12Q 168, C12Q 170, C12P 1934, C07H 2104

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056838751

ABSTRACT:
A method is provided for detecting a target nucleic acid analyte in a sample. The method involves preparing an immobilized capture oligonucleotide that is complementary to the target analyte using a magnetic cycling method, incubating the sample with the immobilized capture oligonucleotide to capture the target analyte thereby forming a capture oligonucleotide-analyte complex, and detecting the presence of the capture oligonucleotide-analyte complex.

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Lund et al. Nucleic Acids Research 16: 10861-10880 1988.

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