Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1989-08-14
1992-07-07
Lilling, Herbert J.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435 6, 435259, 435270, 435820, 536 27, 536 28, 935 19, 935 20, 935 21, C12P 1934, C12N 106, C12N 108, C12N 1110
Patent
active
051282470
ABSTRACT:
The present invention relates to methods and compositons for isolation of nucleic acids from cells. In particular aspects, this invention relates to the use of chaotropic compositions, such as guanidine hydrochloride or guanidinium isothiocyanate, in combination with polyanionic compositions, such as those containing sulfated polysaccharides (i.e., heparin or heparitin sulfate), for the isolation of nucleic acids (RNA or DNA). This method involves disrupting and lysing cells using a nucleic acid releasing composition containing a chaotropic component for the release of nucleic acids from the cell (guanidine hydrochloride or guanidinium isothiocyanate). The released nucleic acids are collected by ethanol precipitation and resuspended before exposure to a polyanion-containing protein dissociating composition which promotes the dissociation of nucleic acid associated proteins from the resuspended nucleic acids. The isolated nucleic acids are washed, further collected by ethanol precipitation and resuspended in a selective buffer prior to further use.
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Board of Regents , The University of Texas System
Lilling Herbert J.
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