Electrophoretic sieving in gel-free media with dissolved polymer

Details Electrophoretic sieving in gel-free media with dissolved polymer Electrophoretic sieving in gel-free media with dissolved polymer

1990-09-27

1992-02-18

Niebling, John

Chemistry: electrical and wave energy

Processes and products

Electrostatic field or electrical discharge

204299R, G01N 2726, B01D 5702

Patent

active

050891111

ABSTRACT:
Separation of sample ions on the basis of molecular size is achieved by electrophoresis in an aqueous solution containing a dissolved non-crosslinked polymer. The polymer has a molecular weight range which overlaps that of the sample ions being separated. Species which vary in molecular weight but not in charge/mass ratio are separated by this method, which is of particular interest in high performance electrophoresis in capillary columns where the use of gels would be awkward and inconvenient.

REFERENCES:
patent: 4305798 (1981-12-01), Cunningham et al.
Hjerten, S. et al, "High-Performance Electrophoresis of Acid and Basic Low-Molecular-Weight Compounds and of Proteins in the Presence of Polymers and Neutral Surfactants", Journal of Liquid Chromatography, 12(13), 2471-2499 (1989).
Tietz, Dietmar et al, "Electrophoresis of Uncrosslinked Polyacrylamide: Molecular Sieving and Its Potential Applications", Electrophoresis 7, 217-220 (1986).

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