Methods for determining peroxidately active substances

Details Methods for determining peroxidately active substances Methods for determining peroxidately active substances

1994-04-22

1995-08-29

Redding, David A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435 25, 435 71, 435 19, 435 18, 435188, 435 4, 436537, 436534, 536 41, 536 172, 536 179, C12Q 128, G01N 33542, G01N 33536

Patent

active

054459443

ABSTRACT:
Methods and compositions are disclosed for determining a peroxidatively active substance (PAS). The methods comprise the step of detecting a fluorescent signal produced upon cleavage of a compound of the formula F-L-Q, wherein F is a fluorescer capable of producing the signal, Q is a quencher capable of quenching the signal when linked to F, and L is a bond, or a linking group having a bond, wherein the bond is capable of being cleaved by a reaction of the PAS with a substrate of the PAS and a hydrogen donor wherein the cleavage of the bond substantially reduces the quenching. The methods have application in a wide variety of systems including assays and improved assays for analytes. Also disclosed are kits for conducting the methods and improvements in accordance with the present invention.

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