Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Patent
1994-04-08
1995-08-29
Beisner, William H.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
435 25, 436111, 436112, 436113, 546119, 546121, C12Q 126
Patent
active
054459435
ABSTRACT:
The invention concerns a method for the colorimetric determination of NADH by means of enzymatic oxidation with benzyl alcohol dehydrogenase whereby an aromatic nitrosoaniline compound of the general formula I is reduced to a colored quinone diimine ##STR1## in which R.sup.1 denotes hydrogen, halogen, hydroxy, alkoxy, aryloxy, alkylthio, arylthio, alkyl or alkylene substituted if desired by carboxy, PO.sub.3 H.sub.2, dialkylphosphinyl or SO.sub.3 H, amino substituted if desired once or twice by alkyl which in turn can be substituted if desired by hydroxy, PO.sub.3 H.sub.2, SO.sub.3 H or carboxy and R denotes a residue which forms a color by electron conjugation with a quinone diimiine system. The invention also concerns a corresponding agent as well as a chromogenic nitroso compound of formula I as well as its use for the enzymatic colorimetric determination of an analyte.
REFERENCES:
patent: 5122244 (1992-06-01), Hoenes et al.
patent: 5206147 (1993-04-01), Hoenes
patent: 5234818 (1993-08-01), Zimmermann et al.
patent: 5240860 (1993-03-01), Hoenes et al.
Beisner William H.
Boehringer Mannheim GmbH
Gitomer Ralph
LandOfFree
Method for the colorimetric determination of an analyte by means does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for the colorimetric determination of an analyte by means, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for the colorimetric determination of an analyte by means will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1818917