Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1993-09-09
1995-08-29
Saunders, David
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 29, 435 39, C12Q 102, G01N 3350
Patent
active
054459397
ABSTRACT:
The ability of mononuclear leukocytes to respond to standard stimuli is measured based on the expression of activation antigens on mononuclear cell subclasses. In a preferred embodiment, a sample of mononuclear leukocytes is cultured for up to 24 hours with a standard stimulus known to activate such cells. After culturing, aliquots of the cells are incubated with fluorophore-conjugated monoclonal antibodies to antigenic determinants of a particular mononuclear subclass and different fluorophore-conjugated monoclonal antibodies to particular activation antigens. The incubated aliquots are analyzed on a flow cytofluorometer, whereby each cell is illuminated with a particular light (e.g. argon ion laser), which detects and measures forward light scatter, orthogonal light scatter and two different wavelengths of light emitted from the fluorophores. These parameters are used to identify and enumerate the cells of different subclasses present within the mononuclear leukocyte sample, the cells of said subclasses which have been induced to express a particular activation antigen and the quantity of the activation antigen on said cells. An analysis of these enumerations is shown to correlate with the immunoregulatory status of the mononuclear leukocyte immune system. Data generation and analysis can be performed using a flow cytofluorometric apparatus with data and control signal processing to ensure accuracy and reproducibility of the results of the assay.
REFERENCES:
patent: 4284412 (1981-08-01), Hansen et al.
patent: 4381295 (1983-04-01), Kung et al.
patent: 4492752 (1985-01-01), Hoffman et al.
patent: 4520110 (1985-05-01), Stryer et al.
patent: 4599304 (1986-07-01), Lanier et al.
patent: 4607007 (1986-08-01), Lanier et al.
patent: 4677061 (1987-06-01), Rose et al.
patent: 4701408 (1987-10-01), Koestler
patent: 4727020 (1988-02-01), Recktenwald
patent: 4778750 (1988-10-01), Gottlieb
Phillips et al, J. Exp. Med., vol. 159, (Apr. 1984), pp. 993-1008.
Blue et al, Journal of Immunology, vol. 134, No. 4, (Apr. 1985) pp. 2281-2286.
Filpovich et al, Clinical Immunology and Immunopathology, vol. 25, (1982) pp. 21-31.
Ebert et al, Clinical Immunology and Immunopathology, vol. 37, (1985) pp. 283-297.
Haynes et al, "Immune Responses of Human Lymphocytes In Vitro" In: Schwartz, R. S., Progress in Clinical Immunology, vol. 4, (New York, Grune & Stratton, 1980) pp. 23-62.
Farrar et al, "The Lymphokine Cascade: A Systemic Model of Immunoregulation" In: Regulation of the Immune Response, (New York, Karger, 1983), pp. 76-87.
Williams et al, Journal of Immunology, vol. 135, No. 4, (Oct., 1985), pp. 2249-2255.
Rabinovitch et al, Journal of Immunology, vol. 136, No. 8, (Apr. 1986) pp. 2769-2775.
Martin et al, Journal of Immunology, vol. 136, No. 9 (May, 1986) pp. 3282-3287.
Kristensen et al, Immunology Letters, vol. 5, (1982), pp. 59-63.
Meuer et al, Journal of Immunology, vol. 129, No. 3 (Sep., 1982), pp. 1076-1079.
Mizel, Immunology Rev., vol. 63, (1982), pp. 51-72.
Waldman, Science, vol. 232 (May 9, 1986) pp. 727-732.
Dillman et al, Cancer Research, vol. 43, (Jan., 1983) pp. 417-421.
Depper et al, Proc. Nat. Acad. Sci USA, vol. 82 (Jun. 1985) pp. 4230-4234.
Wakasugi et al, Journal of Immunology, vol. 135, No. 1 (Jul., 1985), pp. 321-327.
Dudrhiri et al, Journal of Immunology, vol. 135, No. 3 (Sep., 1985), pp. 1813-1818.
Herrmann et al, J. Exp. Med., vol. 162 (Sep., 1985) pp. 1111-1116.
Welte et al, J. Exp. Med., vol. 160, (Nov. 1984), pp. 1390-1403.
Bettens et al, Immunobiol., vol. 170, (1985), pp. 434-447.
Marti et al, American Journal of Hematology, vol. 20, (1985) pp. 41-52.
Damle et al, Journal of Immunology, vol. 134, No. 1, (Jan., 1985), pp. 235-243.
Fox et al., Journal of Immunology, vol. 134, No. 1 (Jan., 1985) pp. 330-335.
Urdal et al, Proc. Nat. Acad. Sci USA, vol. 81, (Oct. 1984), pp. 6481-6485.
Ashman, "Lymphocyte Activation" in Paul, W. E., Fundamental Immunology, (New York, Raven Press, 1984) pp. 267-300.
Miyasaka et al, Clinical Immunology and Immunopathology, vol. 31, (1984) pp. 109-117.
Scher et al, "Cellular Identification and Separation", in Paul, W. E., Fundamental Immunology, (New York, Raven Press; 1984), pp. 767-775.
Bernard et al, Human Immunology, vol. 11 (1984), pp. 1-10.
Weiss et al, Journal of Immunology, vol. 133, No. 1, (Jul., 1984), pp. 123-128.
Meuer et al, Cell, vol. 36, (Apr., 1984), pp. 897-906.
Merrill et al, Journal of Immunology, vol. 133, No. 4, (Oct., 1984) pp. 1931-1937.
Depper et al, Journal of Immunology, vol. 131, No. 2 (Aug., 1983), pp. 690-696.
Landay et al, Journal of Immunology, vol. 131, No. 6, (Dec., 1983), pp. 2757-2761.
Lanier et al, Journal of Immunology, vol. 131, No. 4, (Oct., 1983), pp. 1789-1796.
Cantrell et al, J. Exp. Med. vol. 158 (Dec., 1983) pp. 1895-1911.
Louie et al, Seminars In Oncology, vol. 7, No. 3 (Sep., 1980) pp. 267-284.
Uchiyama et al, Journal of Immunology, vol. 126, No. 4 (Apr., 1981), pp. 1398-1403.
Penn, Clin. Exp. Immunol., vol. 46, (1981), pp. 459-474.
Chouaib et al, Journal of Immunology, vol. 129, No. 6, (Dec., 1982), pp. 2463-2468.
Alexander et al, Journal of Cell Biology, vol. 93, (Jun. 1982), pp. 981-986.
Hoffman et al, Proc. Nat. Acad. Sci. USA, vol. 77, No. 8, (Aug. 1980), pp. 4914-4917.
Salzman et al, Acta Cytologica, vol. 19, No. 4, (Jul.-Aug., 1975) pp. 374-377.
Gillis et al, Journal of Immunology, vol. 123, No. 4 (Oct., 1979) pp. 1624-1631.
Lipkowitz et al, Journal of Immunology, vol. 132, No. 1, (Jan., 1984), pp. 31-37.
Miyawaki et al, Journal of Immunology, vol. 130, No. 6, (Jun., 1983), pp. 2737-2742.
Lanier et al, Journal of Immunology, vol. 132, No. 1 (Jan., 1984), pp. 151-156.
Harris et al, Medical and Pediatric Oncology, vol. 10, (1982), pp. 185-194.
Bach et al, Clin. Exp. Immunol., vol. 45, (1981) pp. 449-456.
Smolen et al, American Journal of Medicine, vol. 72, (May, 1982), pp. 783-790.
Loken et al, Journal of Histochemistry and Cytochemistry, vol. 25, No. 7, (1977), pp. 899-907.
Lanier et al, Immunological Rev., vol. 74, (1983), pp. 143-160.
Lovett, III, et al, Laboratory Investigation, vol. 50, No. 2, (1984), pp. 115-140.
Lazarovits et al, Journal of Immunology, vol. 133, No. 4, (Oct., 1984), pp. 1857-1862.
Fox et al, Journal of Immunology, vol. 133, No. 3, (Sep., 1984), pp. 1250-1256.
Hercend et al, Human Immunology, vol. 3, (1981), pp. 247-259.
Carney, et al., Functional Properties Of T. Lymphocytes In Cytomegalovirus Mononucleosis, The Journal of Immunology, vol. 130, No. 1, Jan. 1983.
Carney, et al., Analysis of T. Lymphocyte Subsets In Cytomegalovirus The Journal of Immunology, vol. 126, No. 6, Jun. 1981.
Reinherz et al., Abnormalities Of Immunoregulatory T Cells In Disorders Of Immune Function, The New England Journal of Medicine vol. 301, No. 19, Nov. 8, 1979.
Product Brochure: "Anti-Interleukin-2 Receptor (CD 25)", Becton Dickinson Immunocytommetry Systems, Mountain View, Calif., (Dec. 1985), one page.
Lovett, III et al., Lab Investigation 50(2), 115-140 (1984).
Cantrell, D A et al., J. Exp. Med 158, 1895-1911 (1983).
Fox, D A et al., J Immunol 134, 330-335 (1985).
Bucy, J Immunol 137:809-813 (1986).
Reis et al., J Immunol 129:2360-2367 (1982).
Reem et al., Science 225:429-430 (1984).
Hess et al., (I), J. Immunol 128:355-359 (1982).
Buurmein et al., J Immunol. 136:4035-4039 (1986).
Gillis et al. (I), J. Immunol 123:1624-1631 (1979).
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