Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1981-08-24
1983-11-22
Love, Ethel G.
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
536 28, 536 29, C07H 1512, C07H 1700
Patent
active
044170467
ABSTRACT:
A polynucleotide product produced by coupling in the presence of a coupling agent (1) a nucleotide or oligonucleotide having a blocked 5'-hydroxyl group and a 3'-phosphate diester group and (2) a nucleotide or oligonucleotide having a blocked 3'-hydroxyl or a blocked 3'-phosphate diester group and a free 5'-hydroxyl group is recovered from the reaction mixture by addition of an organic solvent or mixture of organic solvents which precipitates the polynucleotide product from the reaction mixture while retaining the coupling agent in solution.
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patent: 3792039 (1974-02-01), Erickson et al.
patent: 4210746 (1980-07-01), Kerr et al.
Sung et al., Can. J. Chem., vol. 60, No. 2, Jan. 15, 1982.
Agarwal et al., Journ. Amer. Chem. Soc. 95(6), pp. 2020-2021, (1973).
Ogilvie et al., Can. Journ. Chem., vol. 48, (1970), pp. 862-864.
Werstiuk et al., Can. Journ. Chem., vol. 54, No. 17, (1976), pp. 2689-2696.
Eli Lilly and Company
Love Ethel G.
Martens William C.
Whale Arthur R.
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