Vitro net bioxynthesis of chlorophyll and grana

Details Vitro net bioxynthesis of chlorophyll and grana Vitro net bioxynthesis of chlorophyll and grana

1974-04-23

1976-01-27

Tanenholtz, Alvin E.

Plant husbandry

Process

195 1, A01G 100

Patent

active

039343693

ABSTRACT:
In vitro net biosynthesis of chlorophyll and grana involving: irradiation of etiolated plant tissue; homogenization, in an environment including available oxygen, of the tissue in a buffer comprising biosynthetic cofactors including coenzyme A, glutathione, potassium, inorganic phosphate, methyl alcohol, magnesium, nicotinamide adenine dinucleotide and adenosine triphosphate; isolation from the homogenate of developing chloroplasts; irradiated incubation of the isolated chloroplasts in the presence of oxygen in another buffer of a constitution similar to that above-described and including .delta.-aminolevulinic acid. Synthesized products are isolated and quantified.

REFERENCES:
hatch et al., Photosynthesis and Photorespiration, Wiley-Interscience, New York, 1971.

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