Fast and specific immobilization of nucleic acids to solid suppo

Details Fast and specific immobilization of nucleic acids to solid suppo Fast and specific immobilization of nucleic acids to solid suppo

1985-02-22

1989-04-04

Warren, Charles F.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

935 78, 536 27, 435 91, 436 94, C12Q 168, C07H 2100, C12P 1934

Patent

active

048186813

ABSTRACT:
A process for synthesizing an oligonucleotide comprising linking a nucleoside phosphate to a solid support, through the heterocyclic moiety of the nucleoside, coupling a mono- or oligonucleotide to the nucleoside phosphate through its phosphate moiety, in at least one step enzymatically lengthening the mono- or oligonucleotide, cleaving the resultant oligonucleotide from the solid support-nucleoside phosphate at the phosphate moiety of the nucleoside, and separating the oligonucleotide. After cleaving and separating the solid support-nucleoside phosphate is recycled for further coupling. Advantageously the solid support-nucleoside phosphate is phosphorylated between separation and recycling.

REFERENCES:
patent: 4282352 (1981-08-01), Imahori et al.
patent: 4401796 (1983-08-01), Itakura
patent: 4419444 (1983-12-01), Quash
patent: 4472572 (1984-09-01), Shizuya
patent: 4474947 (1984-10-01), Hudson et al.
patent: 4474948 (1984-10-01), Hudson et al.
patent: 4591564 (1986-05-01), Watson
Connor, B. J. et al Proc. Natl. Acad. Sci. USA vol. 80, 1983, pp. 278-282.
Maniatis, T. et al Molecular Cloning A laboratory manual Cold Spring Habor Laboratory, N.Y. 1982 p. 148.
Ashley, P. et al, Anal. Biochem. vol. 140, 1984, p. 93-103.
Deibel, M. R. et al, Anal Biochem, vol. 144, 1985, pp. 336-346.
Noyes, B. E. et al, Cell, vol. 5, No. 3, 1975, pp. 301-310.
Chemical Abstracts, vol. 95, 1981, p. 318, abstract No. 164568d, Columbus, OH, U.S.; N. F. Krynetskaya et al.: "Immobilized Tris(deoxyribonucleotides) Which are Primers for Enzymic Synthesis of Oligo- an Poly(deoxyribonucleotides)", & DOKL. AKAD. NAUK. SSSR 1981, 258(5), 1242-1245[Biochem.].

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