Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1985-02-19
1989-04-04
Weimar, Elizabeth C.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 25, 435 34, 435 39, 4351721, 935 77, C12Q 168, C12Q 126, C12Q 106, C12Q 104
Patent
active
048186791
ABSTRACT:
This invention concerns a method for recovering mutant cells which produce a substance in a quantifiably reduced amount relative to wild type cells, and the mutant cells so recovered. The method involves contacting the mutant cells under suitable conditions with a suitable amount of an appropriate detectable compound capable of binding to the substance when it is present in the cells so as to permit the detectable compound to bind to the substance. The amount of the detectable compound bound to the substance in the cells is then detected, and the cells which produce the substance in the quantifiably reduced amount are thereby detected. Such cells which produce the quantifiably reduced amount of the substance are then recovered. This invention is applicable to a wide variety of cell types of various genetic characteristics and should therefore be useful in providing a wide range of useful mutant cells.
REFERENCES:
patent: 4345027 (1982-08-01), Dolbeare
patent: 4649109 (1987-03-01), Perlman
Urlaub, G. et al., "Use of Fluorescence-Activated Cell Sorter to Isolate Mutant Mammalian Cells Deficient in an Internal Protein, Dihydrofolate Reductase"; Somatic Cell and Molecular Genetics; vol. 11, No. 1, (Jan., 1985), pp. 71-77.
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Urlaub, G. et al.; "Deletion of the Diploid Dihydrofolate Reductase Locus from Cultured Mammalian Cells", Cell, vol. 33, (Jun., 1983), pp. 405-412.
Kaufman, R. J. et al.; "Quantitation of Dihydrofolate Reductase in Individual Parental and Methotrexate Resistant Cells"; J. Biol. Chem., vol. 253, No. 16, (1978), pp. 5852-5860.
Miller, A. G. et al.; "Novel Variants in Benzo(.alpha.)pyrene Metabolism"; J. Biol. Chem., vol. 256, No. 5, (1981), pp. 2433-2437.
Chasin Gail U.
Chasin Lawrence A.
The Trustees of Columbia University in the City of New York
Weimar Elizabeth C.
White John P.
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